Decreased immune responses to influenza vaccination in patients with heart failure.
Decreased immune responses to influenza vaccination in patients with heart failure.
复制标题
心力衰竭患者对流感疫苗接种的免疫反应降低。
DOI：
10.1016/j.cardfail.2009.05.007
复制
发表时间：
2009
影响因子：
6
通讯作者：
Fildes,JamesE
中科院分区：
文献类型：
--
作者：
Shaw,StevenM;Williams,SimonG;Yonan,Nizar;Fildes,JamesE
关于我们在心力衰竭（HF）患者中更高的IL-10浓度的发现，虽然在我们的培养模型中IFN-γ和IL-10的来源不仅仅特异于细胞毒性T淋巴细胞（CTL），但从Th 1（IFN-γ）到Th 2（IL-10）细胞因子的转变与CTL活性降低相关。1此外，先前的研究已经表明，在细胞培养模型中测量的IFN-γ：IL-10比率可以预测颗粒酶B水平（Grz B，病毒清除过程中的关键酶），其也与CTL活性和疫苗有效性相关。2，3在我们的研究中，与对照组相比，HF参与者中较低的IFN-γ：IL-10比率可能表明CTL活性降低。IFN-γ和IL-10的比例而不仅仅是它们的来源可能为流感疫苗接种提供有价值的T细胞保护，我们相信Wilcoxon秩和检验是一种有效的统计方法，可以比较两个治疗组之间的连续结局指标，事实上，建议的Mann-Whitney U检验和Wilcoxon秩和检验产生相同的统计结果。4 Shaw博士及其同事还质疑在使用非参数检验时进行对数转换的基本原理，并建议应使用T检验分析对数转换数据。尽管通常会对倾斜数据进行对数转换，以便进行T检验
Regarding our finding of higher IL-10 concentrations in patients with heart failure (HF), while the source of IFN-γ and IL-10 in our culture model is not specific to only cytotoxic T-lymphocytes (CTL), a shift from Th1 (IFN-γ) to Th2 (IL-10) cytokines has been associated with reduced CTL activity. 1 Moreover, a previous study has shown that IFN-γ: IL-10 ratios measured in a cell culture model can be predictive of granzyme B levels (Grz B, a key enzyme in the viral clearing process), which also associate with CTL activity and vaccine effectiveness. 2, 3 The lower IFN-γ: IL-10 ratios in HF participants compared to controls reported in our study may suggest decreased CTL activity. The ratio of IFN-γ and IL-10 rather than just their sources may provide valuable insight into T-cell protection afforded by influenza vaccination.We believe the Wilcoxon rank-sum test is a valid statistical approach to compare the continuous outcomes measures between the two treatment groups, and indeed, both the suggested Mann-Whitney U test and the Wilcoxon rank-sum test produce identical statistical results. 4 Dr. Shaw and colleagues also question the rationale for performing a log transformation when a nonparametric test was used and suggest that log-transformed data should be analyzed using a T-test. Though it is common for skewed data to be log-transformed in order that a T-test may be