Changes of telomere length cause reciprocal changes in the lifespan of mother cells in Saccharomyces cerevisiae
Changes of telomere length cause reciprocal changes in the lifespan of mother cells in Saccharomyces cerevisiae
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DOI：
10.1073/pnas.94.18.9768
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发表时间：
1997-09-02
影响因子：
11.1
通讯作者：
Guarente, LP
中科院分区：
文献类型：
--
作者：
Austriaco, NR;Guarente, LP
芽殖酵母细胞不对称分裂，产生母亲和它的女儿，母细胞具有有限的分裂潜力，称为它们的寿命，其结束于增殖停滞和裂解，在这份报告中，我们突变酿酒酵母中的端粒酶以缩短端粒，并表明，而不是缩短寿命，这导致寿命的显着延长，这种延长需要SIR3基因的产物，一种与端粒结合的沉默机制的重要组成部分。相比之下，在基因型野生型菌株中较长的端粒导致寿命缩短。这些发现表明端粒的长度通过调节酵母基因组中非端粒位置可用的沉默机制的量来决定寿命。
Budding yeast cells divide asymmetrically, giving rise to a mother and its daughter, Mother cells have a limited division potential, called their lifespan, which ends in proliferation-arrest and lysis, In this report we mutate telomerase in Saccharomyces cerevisiae to shorten telomeres and show that, rather than shortening lifespan, this leads to a significant extension in lifespan, This extension requires the product of the SIR3 gene, an essential component of the silencing machinery which binds to telomeres. In contrast, longer telomeres in a genotypically wild-type strain lead to a decrease in lifespan, These findings suggest that the length of telomeres dictates the lifespan by regulating the amount of the silencing machinery available to nontelomeric locations in the yeast genome.