CPR5 is involved in cell proliferation and cell death control and encodes a novel trans membrane protein
CPR5 is involved in cell proliferation and cell death control and encodes a novel trans membrane protein
复制标题
DOI：
10.1016/s0960-9822(01)00590-5
复制
发表时间：
2001-11-27
期刊：
影响因子：
9.2
通讯作者：
Hülskamp, M
中科院分区：
文献类型：
--
作者：
Kirik, V;Bouyer, D;Hülskamp, M
植物对病原体的反应通常是牺牲感染部位的细胞。这种类型的程序性细胞死亡在没有病原体的情况下被拟南芥中的结构性致病基因反应5(Cpr5)突变体模拟，表明CPR5在程序性细胞死亡控制中发挥作用[1]。对两个T-DNA标记的cpr5等位基因的细胞表型的分析表明，cpr5在调节细胞内复制和细胞分裂方面具有额外的作用。在cpr5突变毛状体中，内复制周期在两轮后停止，而不是四轮，毛状体细胞的分支比正常少。最终，cpr5毛状体死亡，细胞核解体，细胞崩溃。同样，叶片的生长比野生型更早停止，并且经常观察到显示自发细胞死亡的区域。CPR5基因的克隆揭示了一种新的跨膜蛋白，其胞浆结构域包含核靶向序列。CPR5在细胞增殖和细胞死亡控制中的双重作用表明这两个过程之间存在调控联系。
Plants often respond to pathogens by sacrificing cells at the infection site. This type of programmed cell death is mimicked by the constitutive pathogene response5 (cpr5) mutant in Arabidopsis in the absence of pathogens, suggesting a role for CPR5 in programmed cell death control [1]. The analysis of the cellular phenotypes of two T-DNA-tagged cpr5 alleles revealed an additional role for CPR5 in the regulation of endoreduplication and cell division. In cpr5 mutant trichomes, endoreduplication cycles stop after two rounds instead of four, and trichome cells have fewer branches than normal. Eventually, cpr5 trichomes die, the nucleus disintegrates, and the cell collapses. Similarly, leaf growth stops earlier than in wild-type, and, frequently, regions displaying spontaneous cell death are observed. The cloning of the CPR5 gene revealed a novel putative transmembrane protein with a cytosolic domain containing a nuclear-targeting sequence. The dual role of CPR5 in cell proliferation and cell death control suggests a regulatory link between these two processes.