Distribution of the Intermedilysin Gene among the Anginosus Group Streptococci and Correlation between Intermedilysin Production and Deep-Seated Infection with Streptococcus intermedius
Distribution of the Intermedilysin Gene among the Anginosus Group Streptococci and Correlation between Intermedilysin Production and Deep-Seated Infection with Streptococcus intermedius
复制标题
心绞痛群链球菌中 Intermedilysin 基因的分布及 Intermedilysin 产生与中间链球菌深部感染的相关性
DOI：
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发表时间：
2000
影响因子：
9.4
通讯作者：
H. Kourai
中科院分区：
文献类型：
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作者：
H. Nagamune;R. Whiley;T. Goto;Yasuko Inai;T. Maeda;J. Hardie;H. Kourai
摘要：研究了人类特异性溶细胞素 intermedilysin 在咽峡炎群链球菌中的分布以及中间链球菌毒素产生和感染的相关性。对 intermedilysin 基因特异的 PCR 和 Southern 杂交表明，该毒素基因仅存在于 S. intermedius 和毒素基因没有同源物分布于 S. anginosus 和 S. constellatus 中。因此，intermedilysin基因可用作S. intermedius的标记基因。此外，人类特异性溶血测定和使用中间素特异性抗体进行的蛋白质印迹清楚地表明，来自深部感染（例如脑和肝脓肿）的分离株中中间素的产生水平比来自正常栖息地（例如牙菌斑）或周围感染部位的菌株更高（分别是6.2至10.2倍）。然而，中间链球菌的其他候选毒力因子，例如硫酸软骨素解聚酶、透明质酸酶和唾液酸酶活性，并未显示出酶活性与分离位点或疾病严重程度之间如此明显的相关性。从这些结果来看，intermedilysin 可能是诱导中间链球菌深部感染的重要致病因素或触发因素。
ABSTRACT The distribution of intermedilysin, a human-specific cytolysin, among the anginosus group streptococci and the correlation of toxin production and infection by Streptococcus intermedius were investigated. PCR and Southern hybridization specific for the intermedilysin gene revealed that the toxin gene exists only inS. intermedius and no homologue to the toxin gene is distributed in S. anginosus and S. constellatus. Thus, the intermedilysin gene is useful as a marker gene of S. intermedius. Moreover, a human-specific hemolysis assay and Western blotting with intermedilysin-specific antibodies clearly demonstrated that the intermedilysin production level in isolates from deep-seated infections, such as brain and liver abscesses, is higher (6.2- to 10.2-fold, respectively) than in strains from normal habitats, such as dental plaque, or from peripheral infection sites. However, other candidate virulence factors of S. intermedius, such as chondroitin sulfate depolymerase, hyaluronidase, and sialidase activities, did not show such a clear correlation between enzymatic activity and isolation sites or disease severity. From these results, intermedilysin is likely to be the pathogenic or triggering factor of significance in inducing deep-seated infections with S. intermedius.