Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal Caco-2 cell monolayers
Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal Caco-2 cell monolayers
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DOI：
10.1021/jf035407c
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发表时间：
2004-05-05
影响因子：
6.1
通讯作者：
Kobayashi, S
中科院分区：
文献类型：
--
作者：
Konishi, Y;Kobayashi, S
绿原酸和咖啡酸在Caco-2细胞中都表现出非饱和转运，而咖啡酸也表现出质子耦合的极化吸收。因此，咖啡酸的吸收效率大于绿原酸。MCT的底物如苯甲酸和醋酸可抑制咖啡酸的极化转运。几乎所有顶端负载的绿原酸和咖啡酸都保留在顶端，跨上皮通量与Caco-2细胞的细胞旁通透性呈负相关。这些结果表明，虽然咖啡酸被单羧酸转运体(MCT)吸收的程度较小，但咖啡酸的转运主要是通过细胞旁扩散。此外，间香豆酸和3-(间羟基苯基)丙酸是绿原酸和咖啡酸在结肠微生物中的主要代谢产物，它们竞争性地抑制已知的MCT底物荧光素的转运。这表明它们的吸收也可能由MCT介导。这些发现证明了MCT介导的酚酸本身及其结肠代谢物吸收的生理重要性。
Both chlorogenic and caffeic acids exhibited nonsaturable transport in Caco-2 cells, whereas caffeic acid also showed proton-coupled polarized absorption. Thus, the absorption efficiency of caffeic acid was greater than that of chlorogenic acid. Polarized transport of caffeic acid was inhibited by substrates of MCT such as benzoic and acetic acids. Almost all of the apically loaded chlorogenic and caffeic acid was retained on the apical side, and the transepithelial flux was inversely correlated with the paracellular permeability of Caco-2 cells. These results indicate that transport was mainly via paracellular diffusion, although caffeic acid was absorbed to a lesser extent by the monocarboxylic acid transporter (MCT). Furthermore, m-coumaric acid and 3-(m-hydroxyphenyl)propionic acid, the main metabolites of chlorogenic and caffeic acid by colonic microflora, competitively inhibited the transport of fluorescein, a known substrate of MCT. This suggests that their absorption could also be mediated by MCT. These findings have exemplified the physiological importance of MCT-mediated absorption in both phenolic acids per se and their colonic metabolites.