Engineering Saccharomyces cerevisiae Coculture Platform for the Production of Flavonoids
Engineering Saccharomyces cerevisiae Coculture Platform for the Production of Flavonoids
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用于生产类黄酮的工程酿酒酵母共培养平台
DOI：
10.1021/acs.jafc.9b07916
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发表时间：
2020-02-19
影响因子：
6.1
通讯作者：
Li, Mu
中科院分区：
文献类型：
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作者：
Du, Yun;Yang, Binrui;Li, Mu
黄酮类化合物是一种有价值的天然产物，在人体健康和营养方面有着广泛的应用。工程菌群表达复杂的类黄酮生物合成途径是一种很有前途的类黄酮生产途径。在本研究中，整个类黄酮生物合成途径被分成两个独立的途径，每个途径都包含在单独的酿酒酵母细胞中。通过基因工程表达柚皮素生物合成途径的第一种细胞类型sNAR5产生了144.1 mg/L的柚皮素。第二种细胞类型采用外源柚皮素-水飞蓟素途径进行基因修饰。共培养产生了飞燕素滴度，显著高于菌株sDPD2的单一培养，包含了整个途径。此外，我们成功地利用该共培养平台在瓶级培养中生产了3种黄酮醇和2种花青素。该共培养平台为开发经济高效的微生物类黄酮生产工艺铺平了道路。
Flavonoids are valuable natural products widely used in human health and nutrition applications. Engineering microbial consortia to express complex flavonoid biosynthetic pathways is a promising approach for flavonoid production. In this study, the entire flavonoid biosynthetic pathway was split into two independent pathways, each of which was contained in separate Saccharomyces cerevisiae cells. The first cell type, sNAR5, which was genetically engineered to express the naringenin biosynthetic pathway, produced 144.1 mg/L naringenin. The second cell type was genetically modified with the heterologous naringenin-todelphinidin pathway. A coculture produced a delphinidin titer, significantly higher than that produced in a monoculture of strain sDPD2, harboring the entire pathway. Furthermore, we successfully employed this coculture platform for the production of 3 flavonols and 2 anthocyanidins in flask-scale culture. This coculture platform paves the way for the development of an economical and efficient process for microbial flavonoid production.