PRODUCTION OF EXOPOLYSACCHARIDES BY A PROBIOTIC STRAIN OF LACTOBACILLUS RHAMNOSUS: BIOSYNTHESIS AND PURIFICATION METHODS
PRODUCTION OF EXOPOLYSACCHARIDES BY A PROBIOTIC STRAIN OF LACTOBACILLUS RHAMNOSUS: BIOSYNTHESIS AND PURIFICATION METHODS
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DOI：
10.1556/aalim.42.2013.2.9
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发表时间：
2013-06-01
期刊：
影响因子：
1.1
通讯作者：
Kreft, A.
中科院分区：
文献类型：
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作者：
Polak-Berecka, M.;Wasko, A.;Kreft, A.
产生胞外多糖的能力在乳酸菌中广泛存在；然而，只有部分菌株能同时产生荚膜多糖（CPS）和胞外多糖（EPS）。对新型合成产品日益增长的需求要求寻找新的益生菌eps生产菌株。在这项工作中，我们提供的证据表明，鼠李糖乳杆菌E/N能够产生两种形式的多糖，当生长在MRS肉汤。通过光学显微镜和透射电镜观察细菌表面周围的CPS。生长培养基中存在的EPS主要在指数生长阶段产生。从鼠李糖E/N培养基中提取CPS和EPS，产量分别为12.6 mg l（-1）和146 mg l（-1）。结果表明，分离EPS馏分的方法对EPS的最终收率有很大影响。本研究提出了从鼠李糖培养物中提取EPS的最有效方法。鼠李糖E/N作为一种益生菌具有潜在的技术应用价值，可用于改善新药品的性能。
The ability to produce exocellular polysaccharides is widespread among lactic acid bacteria; however, only some strains produce both capsular polysaccharides (CPS) and exopolysaccharides (EPS). The increasing demand for novel synbiotic products requires a search for new probiotic EPS-producing strains. In this work, we provide evidence that Lactobacillus rhamnosus E/N is capable of producing both forms of polysaccharides when grown in MRS broth. CPS surrounding the bacterial surface was observed by optical microscopy and transmission electron microscopy. EPS present in the growth medium was produced mainly during the exponential growth phase. CPS and EPS were extracted from the culture of Lb. rhamnosus E/N with yields of 12.6 mg l(-1) and 146 mg l(-1), respectively. It was shown that the method used to isolate the EPS fraction exerted a strong influence on the final amount of EPS obtained. In this study, we propose the most effective method of extraction of EPS from Lb. rhamnosus cultures. Lb. rhamnosus E/N could be considered for potential technological application as a probiotic for use in improving the properties of new pharmaceutical products.