Identification of a cardiac isoform of the murine calcium channel α1C (Cav1.2-a) subunit and its preferential binding with the β2 subunit
Identification of a cardiac isoform of the murine calcium channel α1C (Cav1.2-a) subunit and its preferential binding with the β2 subunit
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DOI：
10.1016/j.yjmcc.2006.05.002
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发表时间：
2006-07-01
影响因子：
5
通讯作者：
Iijima, Toshihiko
中科院分区：
文献类型：
--
作者：
Murakami, Manabu;Ohba, Takayoshi;Iijima, Toshihiko
我们描述了一个心肌亚型的电压依赖性钙通道α 1亚基，这对应于兔直系同源的α 1C-a（Ca（v）1.2a）。我们还克隆了平滑肌亚型α 1C-b（Ca（v）1.2b）和α 1C-d（Ca（v）1.2d）。这三种亚型之间的差异在于N-末端区域（外显子1A或1B）、结构域1的第六个跨膜区段（外显子8A或8B）以及外显子10的使用，外显子10在跨膜结构域I和II之间形成胞内环。双杂交分析揭示了三种α 1亚型和β亚基之间的相互作用。体外覆盖和免疫沉淀分析显示，α 1C-α和β 2之间优先结合，β 2在心脏中也以高水平表达。(c)2006年爱思唯尔公司All rights reserved.
We describe a cardiac muscle isoform of the voltage-dependent calcium channel alpha 1 subunit, which corresponds to the rabbit ortholog of alpha 1C-a (Ca(v)1.2a). We also cloned smooth muscle isoforms alpha 1C-b (Ca(v)1.2b) and alpha 1C-d (Ca(v)1.2d). Differences among these three isoforms lie within the N-tenninal region (exon 1A or 1B), the sixth transmembrane segment of domain 1 (exon 8A or 8B), and the use of exon 10, which forms the intracellular loop between transmembrane domains I and II. Two-hybrid analysis revealed interactions among the three alpha 1 isoforms and beta subunits. In vitro overlay and immunoprecipitation analyses revealed preferential binding between alpha 1C-alpha and beta(2), which is also expressed at a high level in the heart. (c) 2006 Elsevier Inc. All rights reserved.