Expression profiling of the host response to bacterial infection:: the transition from basal to induced defence responses in RPM1-mediated resistance
Expression profiling of the host response to bacterial infection:: the transition from basal to induced defence responses in RPM1-mediated resistance
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DOI：
10.1046/j.1365-313x.2003.01653.x
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发表时间：
2003-02-01
期刊：
影响因子：
7.2
通讯作者：
Grant, M
中科院分区：
文献类型：
--
作者：
de Torres, M;Sanchez, P;Grant, M
拟南芥叶片中的转录变化的特点是挑战与菌株的假单胞菌pv。番茄DC3000允许区分基础抗性（hrpA突变体）、基因特异性抗性（RPM1特异性相互作用）和易感性（野生型病原体）。在植物无毒基因诱导中，宿主[Ca（2+）]（cyt）的变化和叶片塌陷被用来描述从感染到诱导抗性的转变。植物对植物病原细菌的感染反应迅速、动态和有区别。在前2小时内，宿主转录变化对于所有挑战都是常见的，表明III型效应子功能对宿主转录组重编程中的早期事件没有贡献。特定转录物的诱导时间是可重复的，分层的，并且至少部分地通过EDS1功能进行调节。R基因特异性转录物直到接种后3 h才被观察到。有趣的是，R基因特异性反应蛋白预计将定位于不同的细胞地址，表明对细胞稳态的全球影响。改变的转录反应迅速表现为2小时内叶萎陷的初始症状，尽管完全宏观HR的建立明显较晚。
Changes in transcription in leaves of Arabidopsis thaliana were characterised following challenge with strains of Pseudomonas syringae pv. tomato DC3000 allowing differentiation of basal resistance (hrpA mutants), gene-specific resistance (RPM1 -specified interactions) and susceptibility (wild-type pathogen). In planta avirulence gene induction, changes in host [Ca(2+) ](cyt) and leaf collapse were used to delineate the transition from infection to induced resistance. The plant responds rapidly, dynamically and discriminately to infection by phytopathogenic bacteria. Within the first 2 h host transcriptional changes are common to all challenges indicating that Type III effector function does not contribute to early events in host transcriptome re-programming. The timing of induction for specific transcripts was reproducible, hierarchical and modulated at least in part through EDS1 function. R gene-specific transcripts were not observed until 3 h after inoculation. Intriguingly, the R gene-specific response proteins are expected to localise to diverse cellular addresses indicative of a global impact on cellular homeostasis. The altered transcriptional response rapidly manifests into initial symptoms of leaf collapse within 2 h, although establishment of the full macroscopic HR occurs significantly later.