Context-dependent sensitization to ethanol in zebrafish (Danio rerio)
Context-dependent sensitization to ethanol in zebrafish (Danio rerio)
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DOI：
10.1016/j.pbb.2010.02.002
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发表时间：
2010-05-01
影响因子：
3.6
通讯作者：
Poliner, Rebecca M.
中科院分区：
文献类型：
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作者：
Blaser, Rachel E.;Koid, Audrey;Poliner, Rebecca M.
中等剂量的乙醇对斑马鱼行为的最强大和最容易测量的影响之一是运动过度活跃。设计了两个实验来研究重复暴露于乙醇对乙醇诱导的运动过度活动的影响，并确定这些影响是否是上下文依赖的。成年野生型斑马鱼在一种环境刺激存在下重复暴露于乙醇（A），而在第二种环境刺激存在下暴露于水（B）。暴露于乙醇始终诱导运动过度活跃。重复暴露后，在乙醇配对环境中用乙醇测试的动物（A）显示出运动活动的致敏性。然而，当在未配对的情况下（B）用乙醇进行试验时，未观察到致敏性。当在没有乙醇的情况下进行测试时，对配对和非配对刺激的反应没有差异。这是第一次证明乙醇诱导的斑马鱼运动敏化。此外，这种敏化是特定于上下文的，表明学习可以改变斑马鱼中药物诱导的行为。(C)2010年爱思唯尔公司All rights reserved.
One of the most robust and readily measurable effects of moderate doses of ethanol on zebrafish behavior is locomotor hyperactivity. Two experiments were designed to examine the effects of repeated exposures to ethanol on ethanol-induced locomotor hyperactivity, and to determine whether these effects are context-dependent. Adult, wild-type zebrafish were given repeated exposure to ethanol in the presence of one contextual stimulus (A), while exposed to water in the presence of a second contextual stimulus (B). Exposure to ethanol consistently induced locomotor hyperactivity. After repeated exposures, animals tested with ethanol in the ethanol-paired context (A) showed sensitization of locomotor activity. When tested with ethanol in the unpaired context (B), however, sensitization was not observed. When tested in the absence of ethanol, there were no differences in responding to the paired and unpaired stimuli. This is the first demonstration of ethanol-induced locomotor sensitization in zebrafish. Moreover, this sensitization was context-specific, indicating that learning can modify drug-induced behaviors in zebrafish. (C) 2010 Elsevier Inc. All rights reserved.