Large-Scale Single Particle and Cell Trapping based on Rotating Electric Field Induced-Charge Electroosmosis.
Large-Scale Single Particle and Cell Trapping based on Rotating Electric Field Induced-Charge Electroosmosis.
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DOI：
10.1021/acs.analchem.6b03413
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发表时间：
2016-11
影响因子：
7.4
通讯作者：
Yupan Wu;Yukun Ren;Ye Tao;Likai Hou;Hongyuan Jiang
中科院分区：
文献类型：
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作者：
Yupan Wu;Yukun Ren;Ye Tao;Likai Hou;Hongyuan Jiang
我们提出了一种简单，廉价的微流控芯片大规模捕获的单个颗粒和细胞的基础上诱导电荷电渗在旋转电场（ROT-ICEO）。中心浮动电极阵列被放置在具有正交配置的四个驱动电极之间的差距的中心，并用于固定单个颗粒或细胞。通过ROT-ICEO流和浮力流之间的相互作用将细胞捕获在电极阵列上。我们通过研究颗粒或细胞密度和电势等重要参数，实验优化了捕获单个颗粒的效率。实验和数值计算结果表明，良好的协议。通过捕获单个直径为5 μm和20 μm的聚苯乙烯（PS）微球和单个酵母细胞，验证了芯片的工作性能。当微球直径为5 μm、密度为800个/μL时，采用直径为20 μm的浮动电极阵列，幅值电压为5 V，频率为10 kHz，可获得最高的单粒子占有率，为73%。ROT-ICEO流可以以低于0.45 μL/min的速率保持细胞对流体流动。这种新颖、简单、稳健的捕获单细胞的方法在遗传和代谢工程中具有巨大的潜力。
We propose a simple, inexpensive microfluidic chip for large-scale trapping of single particles and cells based on induced-charge electroosmosis in a rotating electric field (ROT-ICEO). A central floating electrode array, was placed in the center of the gap between four driving electrodes with a quadrature configuration and used to immobilize single particles or cells. Cells were trapped on the electrode array by the interaction between ROT-ICEO flow and buoyancy flow. We experimentally optimized the efficiency of trapping single particles by investigating important parameters like particle or cell density and electric potential. Experimental and numerical results showed good agreement. The operation of the chip was verified by trapping single polystyrene (PS) microspheres with diameters of 5 and 20 μm and single yeast cells. The highest single particle occupancy of 73% was obtained using a floating electrode array with a diameter of 20 μm with an amplitude voltage of 5 V and frequency of 10 kHz for PS microbeads with a 5-μm diameter and density of 800 particles/μL. The ROT-ICEO flow could hold cells against fluid flows with a rate of less than 0.45 μL/min. This novel, simple, robust method to trap single cells has enormous potential in genetic and metabolic engineering.