Nucleotide sequence of a chromosomal rearranged lambda 2 immunoglobulin gene of mouse
Nucleotide sequence of a chromosomal rearranged lambda 2 immunoglobulin gene of mouse
复制标题
小鼠染色体重排的lambda 2免疫球蛋白基因的核苷酸序列
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
H. Murialdo
中科院分区：
文献类型：
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作者：
Gillian E. Wu;N. Govindji;Nobumichi Hozumi;H. Murialdo
克隆了小鼠浆细胞瘤细胞系MOPC 315的重排λ 2基因并测定了其序列。将其序列与未重排的（生殖系）V、J和C基因片段的序列进行比较，表明V基因片段的序列在6个位置上不同。J和C基因片段的序列保持不变。这些结果支持了免疫球蛋白基因中发生体细胞突变的假设，这些突变不涉及C基因片段。λ 2基因的元件与λ 1基因以及C λ 3和C λ 4基因片段的元件的同源性程度提示了通过免疫球蛋白λ轻链基因座的基因复制的进化途径。根据该方案，原始结构V0-J 0 C 0通过J 0 C 0区域的复制产生结构V0-J1 C1-J11 C11。第二次重复覆盖整个区域，形成了目前的结构：V1-J3 C3-J1 C1/V2-J2 C2-J 4C 4。
The rearranged lambda 2 gene of the mouse plasmacytoma cell line MOPC315 has been cloned and sequenced. A comparison of its sequence with the sequence of the unrearranged (germ-line) V, J and C gene segments shows that the sequences of the V gene segments differ at six positions. The sequence of the J and C gene segments remained unchanged. These results add support to the hypothesis that somatic mutations occur in immunoglobulin in genes and that these mutations do not involve the C gene segment. The degree of homology of the elements of the lambda 2 gene with those of the lambda 1 gene and C lambda 3 and C lambda 4 gene fragments suggest a pathway of evolution by gene duplication of the immunoglobulin lambda light chain locus. According to this scheme the original structure V0-J0C0 gave rise to a structure V0-J1C1-J11C11 by duplication of the J0C0 region. A second duplication encompassing the whole region resulted in the present structure: V1-J3C3-J1C1/V2-J2C2-J4C4.