Plasma cardiac troponin T closely correlates with infarct size in a mouse model of acute myocardial infarction
Plasma cardiac troponin T closely correlates with infarct size in a mouse model of acute myocardial infarction
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DOI：
10.1016/s0009-8981(02)00296-6
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发表时间：
2002-11-01
影响因子：
5
通讯作者：
Mair, J
中科院分区：
文献类型：
--
作者：
Metzler, B;Hammerer-Lercher, A;Mair, J
背景资料：心肌肌钙蛋白取代肌酸激酶和乳酸脱氢酶同工酶作为心肌损伤的实验室诊断标准。然而，心肌肌钙蛋白T（cTnT）与病理学确定的梗死面积的相关性只有少数出版物，这是有缺陷的样本量不足。方法：38只野生型小鼠麻醉后，开胸、心包，结扎左降支动脉。假手术后，所有小鼠均恢复正常。急性心肌梗死手术诱导后24小时，采集肝素化血样，处死动物并收获心脏。使用Cardiac Reader（Roche Diagnostics）通过定量快速测定法测量血浆cTnT。将心脏切成四个横切面，用1.5%氯化三苯基四唑染色，通过计算机面积测定法评价坏死。计算组织学梗死面积和心脏标志物之间的Pearson相关系数。结果：梗死面积范围为左心室的14%至55%，心肌肌钙蛋白T浓度为3.9至14.1 μ g/l。cTnT与组织学梗死面积密切相关（r=0.84，p
Background: Cardiac troponins replaced creatine kinase and lactate dehydrogenase isoenzymes as criterion standards for the laboratory diagnosis of myocardial damage. However, there are only few publications on correlations of cardiac troponin T (cTnT) with pathologically determined infarct size which are flawed by insufficient sample size. Methods: In 38 anesthetized wildtype mice, the chest and pericardium were opened and the left descending artery was ligated. After sham operation, all mice recovered. Twenty-four hours after surgical induction of acute myocardial infarction heparinized blood samples were collected, the animals sacrificed and hearts harvested. Plasma cTnT was measured by a quantitative rapid assay using the Cardiac Reader (Roche Diagnostics). The hearts were cut into four transverse sections which were stained with 1.5% triphenyltetrazolium chloride for evaluation of necrosis by computerized planimetry. Pearson correlation coefficients were calculated between histological infarct size and cardiac markers. Results: Infarct sizes ranged from 14% to 55% of left ventricle and cTnT concentrations from 3.9 to 14.1 mug/l. cTnT correlated closely with histological infarct size (r=0.84, p