Metabolic costs of mounting an antigen-stimulated immune response in adult and aged C57BL/6J mice
Metabolic costs of mounting an antigen-stimulated immune response in adult and aged C57BL/6J mice
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DOI：
10.1152/ajpregu.1997.273.5.r1631
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发表时间：
1997-11-01
影响因子：
2.8
通讯作者：
Nelson, RJ
中科院分区：
文献类型：
--
作者：
Demas, GE;Chefer, V;Nelson, RJ
动物必须平衡它们的能量预算，尽管能量可用性和生理消耗的季节变化。除了生长、体温调节和细胞维持外，免疫还需要大量的能量来维持功能，尽管很少有研究直接测试免疫的能量成本。目前的研究评估了抗体反应的代谢成本。将成年和老年雄性C5BL/6J小鼠分别植入空硅胶胶囊或填充褪黑素胶囊，并注射生理盐水或锁孔帽贝血青素（KLH）。在抗体生产过程中，使用间接量热法定期监测O-2消耗。与注射生理盐水的小鼠相比，注射klh的小鼠表现出明显的免疫球蛋白G （IgG）反应，消耗更多的O-2。褪黑素治疗增加了生理盐水注射小鼠的O-2消耗，但抑制了与klh注射动物免疫反应相关的代谢率的增加。褪黑素对KLH的免疫应答无影响。成年小鼠和老年小鼠在抗体反应或代谢活性方面没有差异，尽管老年小鼠的O-2产量与成年小鼠相当，但它们似乎无法维持足够的产热。这些结果表明，建立免疫反应需要大量的能量，因此需要使用本来可以分配给其他生理过程的资源。当能量需求高时（例如，在冬季，怀孕或哺乳期），可能会进行能量权衡。褪黑素似乎在协调生殖、免疫和能量过程中起适应性作用。
Animals must balance their energy budget despite seasonal changes in both energy availability and physiological expenditures. Immunity, in addition to growth, thermoregulation, and cellular maintenance, requires substantial energy to maintain function, although few studies have directly tested the energetic cost of immunity. The present study assessed the metabolic costs of an antibody response. Adult and aged male C5BL/6J mice were implanted with either empty Silastic capsules or capsules filled with melatonin and injected with either saline or keyhole limpet hemocyanin (KLH). O-2 consumption was monitored periodically throughout antibody production using indirect calorimetry. KLH-injected mice mounted significant immunoglobulin G (IgG) responses and consumed more O-2 compared with animals injected with saline. Melatonin treatment increased O-2 consumption in mice injected with saline but suppressed the increased metabolic rate associated with an immune response in KLH-injected animals. Melatonin had no effect on immune response to KLH. Adult and aged mice did not differ in antibody response or metabolic activity Aged mice appear unable to maintain sufficient heat production despite comparable O-2 production to adult mice. These results suggest that mounting an immune response requires significant energy and therefore requires using resources that could otherwise be allocated to other physiological processes. Energetic trade-offs are likely when energy demands are high (e.g., during winter, pregnancy, or lactation). Melatonin appears to play an adaptive role in coordinating reproductive, immunologic, and energetic processes.