Polyphosphate, derived from the probiotic Lactobacillus brevis, modulates the intestinal microbiome and attenuates acute pancreatitis.
Polyphosphate, derived from the probiotic Lactobacillus brevis, modulates the intestinal microbiome and attenuates acute pancreatitis.
复制标题
多磷酸盐源自益生菌短乳杆菌，可调节肠道微生物组并减轻急性胰腺炎。
DOI：
10.1007/s10620-020-06747-9
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Okumura T.
中科院分区：
文献类型：
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作者：
Takauji S;Konishi H;Fujiya M;Ueno N;Tanaka H;Sato H;Isozaki S;Kashima S;Moriichi K;Mizukami Y;Okumura T.
背景我们以前发现短乳杆菌衍生的多聚磷酸盐（poly P）对肠道炎症有治疗作用。然而，聚P是否改善炎症和损伤的远端organs.AimsWe的目的是调查肠道微生物的变化，并评估聚P的保护作用对损伤的雨蛙肽诱导的急性胰腺炎（AP）mouse.MethodsPoly P每天口服给药BALB/C小鼠24天，然后小鼠腹腔注射雨蛙肽。在注射雨蛙肽之前，收集粪便样品并通过16 S rRNA基因测序进行分析。小鼠被处死后24小时，最后雨蛙素注射;随后，血清，胰腺，结肠collected.ResultsThe微生物的配置文件之间的聚P和对照组显着不同。值得注意的是，在聚P组中，有益细菌（包括Alistipes和Escherichidatus_Escherichimonas）的水平显著增加，而毒性细菌脱硫弧菌的水平降低。血清淀粉酶和脂肪酶水平的雨蛙肽治疗的升高被抑制的聚P的预管理24天，但不是7天。AP + Poly P组MPO阳性细胞数明显减少，MCP-1水平明显降低。免疫荧光分析表明，ZO-1和occludin在结肠中的AP + Poly P group.ConclusionsPoly P减弱AP通过修改肠道微生物组和增强肠道屏障的完整性的上皮细胞膜层强烈增强。
BackgroundWe previously showed thatLactobacillus brevis-derived polyphosphate (poly P) exerts a curative effect on intestinal inflammation. However, whether or not poly P improves the inflammation and injury of distant organs remains unclear.AimsWe aimed to investigate the change in the intestinal microbiome and to evaluate the protective effect of poly P on injuries in a cerulein-induced acute pancreatitis (AP) mouse.MethodsPoly P was orally administered to BALB/C mice every day for 24 days, and then mice were intraperitoneally injected with cerulein. Before cerulein injection, stool samples were collected and analyzed by 16S rRNA gene sequencing. Mice were sacrificed at 24 h after the last cerulein injection; subsequently, the serum, pancreas, and colon were collected.ResultsThe microbial profile differed markedly between poly P and control group. Notably, the levels of beneficial bacteria, includingAlistipesandCandidatus_Saccharimonas, were significantly increased, while those of the virulent bacteriaDesulfovibriowere decreased in the poly P group. The elevations of the serum amylase and lipase levels by cerulein treatment were suppressed by the pre-administration of poly P for 24 days, but not for 7 days. The numbers of cells MPO-positive by immunohistology were decreased and the levels of MCP-1 significantly reduced in the AP + Poly P group. An immunofluorescence analysis showed that the ZO-1 and occludin in the colon was strongly augmented in the epithelial cell membrane layer in the AP + Poly P group.ConclusionsPoly P attenuates AP through both modification of the intestinal microbiome and enhancement of the intestinal barrier integrity.