STIMULATION OF DNA-SYNTHESIS AND MITOSIS OF HEPATOCYTES IN PRIMARY CULTURES OF NEONATAL RAT-LIVER BY ARACHIDONIC-ACID AND PROSTAGLANDINS
STIMULATION OF DNA-SYNTHESIS AND MITOSIS OF HEPATOCYTES IN PRIMARY CULTURES OF NEONATAL RAT-LIVER BY ARACHIDONIC-ACID AND PROSTAGLANDINS
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DOI：
10.1016/0014-4827(81)90425-0
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发表时间：
1981-01-01
影响因子：
3.7
通讯作者：
ARMATO, U
中科院分区：
文献类型：
--
作者：
ANDREIS, PG;WHITFIELD, JF;ARMATO, U
At low concentrations (10-12-10-9 mol/l) arachidonic acid intensely stimulated both DNA synthetic and mitotic activities of hepatocytes in 4 day old primary cultures of neonatal rat liver. This effect of arachidonate was completely suppressed by the simultaneous administration to the cultures of a high dose (10-4 mol/l) of indomethacin. A similar, but much weaker proliferogenic activity was exerted on neonatal hepatocytes by quite low concentrations of some of the main products of arachidonic acid metabolism, namely prostaglandin [PG] A1, PGE1, and PGE2. Although these data support the possibility that arachidonate and PG are involved in the regulation of hepatocytic proliferative activation, the exact role of PG remains to be ascertained because such agents may have acted by inducing intracellular surges of known mitogenic compounds, such as cAMP and cGMP.
