The long-term effect of glutaraldehyde on the bacterial community in anaerobic ammonium oxidation reactor.
The long-term effect of glutaraldehyde on the bacterial community in anaerobic ammonium oxidation reactor.
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DOI：
10.1016/j.biortech.2023.129448
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发表时间：
2023-07
影响因子：
11.4
通讯作者：
Longyu Jiang;Lu Guo;J. Shapleigh;Yi Liu;Yan Huang;Jinshan Lian;Ling Xie;Liang-wei Deng;Wenguo Wang;Lan Wang
中科院分区：
文献类型：
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作者：
Longyu Jiang;Lu Guo;J. Shapleigh;Yi Liu;Yan Huang;Jinshan Lian;Ling Xie;Liang-wei Deng;Wenguo Wang;Lan Wang
用两个厌氧氨氧化反应器（GA和CK）进行了160天的培养，以研究戊二醛的影响。结果表明，当GA反应器中的戊二醛浓度增加到40 mg/L时，厌氧氨氧化菌非常敏感，脱氮效率急剧下降至11%，仅为对照的四分之一。戊二醛改变了胞外多糖的空间分布，导致厌氧氨氧化菌（BrocadiaCK_gra75）从颗粒中解离（CK中的24.70%，而GA颗粒中仅14.09%）。宏基因组分析表明，戊二醛导致了无nir（亚硝酸盐还原酶）和nor（一氧化氮还原酶）基因的菌株向有nir和nor基因的菌株的群落演替，以及具有NodT（一种外膜因子）相关外排泵的菌株的快速生长，所述外排泵被另一种TolC相关外排泵取代。同时，BrocadiaCK_gra75缺乏NodT蛋白。本研究为了解活性厌氧氨氧化菌群落在消毒剂作用下的适应性和潜在抗性机制提供了重要依据。
A 160-day incubation was performed with two anammox reactors (GA and CK) to investigate the effect of glutaraldehyde. The results indicated that anammox bacteria were very sensitive when glutaraldehyde in GA reactor increased to 40 mg/L, the nitrogen removal efficiency sharply decreased to 11%, only one-quarter of CK. Glutaraldehyde changed spatial distribution of exopolysaccharides, caused anammox bacteria (BrocadiaCK_gra75) to disassociate from granules (24.70% of the reads in CK but only 14.09% in GA granules). Metagenome analysis indicated glutaraldehyde led to the denitrifier community succession from strains withoutnir(nitrite reductase) and nor (nitric oxide reductases) genes to those with them, and the rapid growth of denitrifiers with NodT (an outer membrane factor)-related efflux pumps replacing those with another TolC -related ones. Meanwhile,BrocadiaCK_gra75 lacks the NodT proteins. This study provides important insight into community adaptation and potential resistance mechanism in an active anammox community after exposure to disinfectant.