The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growth in vivo (Retracted article. See vol. 89B, pg. 575, 2009)
The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growth in vivo (Retracted article. See vol. 89B, pg. 575, 2009)
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DOI：
10.1002/jbm.b.30713
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发表时间：
2007-07-01
影响因子：
3.4
通讯作者：
Knight, Kenneth R.
中科院分区：
文献类型：
--
作者：
Cronin, Kevin J.;Messina, Aurora;Knight, Kenneth R.
在体内小鼠血管化室模型已被证明产生自发血管生成和新组织形成。本实验旨在评估常用生物支架对该模型组织生长的影响。将层粘连蛋白-1、I型胶原蛋白、纤维蛋白胶、透明质酸或海绵分别插入含有腹壁动静脉的硅胶腔中，一端用脂肪组织密封，另一端用骨蜡密封，然后皮下孵育。2周、4周或6周后，含有胶原蛋白1、纤维蛋白胶、透明质酸或未添加支架（对照）的腔室组织中有少量血管化结缔组织。含有海绵的腔室有中度结缔组织生长，并伴有轻度“异物”炎症反应。含有层粘连蛋白-1的腔室，其浓度比其在Matrigel （TM）中的浓度低10倍，导致中度脂肪生成反应。综上所述，(1)生物水凝胶被吸收并逐渐被血管化结缔组织所取代；(2)具有大孔隙的海绵状基质支持孔隙内结缔组织生长，并被肉芽组织包裹；(3)含层粘连蛋白支架促进脂肪形成。由此可见，支架的性质和化学成分对该体内腔室模型中生成的组织的数量和类型有显著影响。(c) 2006 Wiley期刊公司
An in vivo murine vascularized chamber model has been shown to generate spontaneous angiogenesis and new tissue formation. This experiment aimed to assess the effects of common biological scaffolds on tissue growth in this model. Either laminin-1, type I collagen, fibrin glue, hyaluronan, or sea sponge was inserted into silicone chambers containing the epigastric artery and vein, one end was sealed with adipose tissue and the other with bone wax, then incubated subcutaneously. After 2,4, or 6 weeks, tissue from chambers containing collagen 1, fibrin glue, hyaluronan, or no added scaffold (control) had small amounts of vascularized connective tissue. Chambers containing sea sponge had moderate connective tissue growth together with a mild "foreign body" inflammatory response. Chambers containing laminin-1, at a concentration 10-fold lower than its concentration in Matrigel (TM), resulted in a moderate adipogenic response. In summary, (1) biological hydrogels are resorbed and gradually replaced by vascularized connective tissue; (2) sponge-like matrices with large pores support connective tissue growth within the pores and become encapsulated with granulation tissue; (3) laminin-containing scaffolds facilitate adipogenesis. It is concluded that the nature and chemical composition of the scaffold exerts a significant influence on the amount and type of tissue generated in this in vivo chamber model. (c) 2006 Wiley Periodicals, Inc.