GmCLC1 Confers Enhanced Salt Tolerance through Regulating Chloride Accumulation in Soybean.
GmCLC1 Confers Enhanced Salt Tolerance through Regulating Chloride Accumulation in Soybean.
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DOI：
10.3389/fpls.2016.01082
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发表时间：
2016
影响因子：
5.6
通讯作者：
Lam HM
中科院分区：
文献类型：
--
作者：
Wei P;Wang L;Liu A;Yu B;Lam HM
氯离子通道蛋白家族介导氯离子的运输，在植物的营养供应、细胞动作电位和膨压调节、气孔运动、激素信号识别和转导、氯离子稳态以及非生物和生物胁迫耐受等方面发挥重要作用。盐胁迫下，主要由氯离子（Cl-）引起的阴离子毒性对植物的影响还不清楚。本研究以转基因拟南芥、大豆和酵母为材料，研究了大豆Cl-/H+逆向转运蛋白GmCLC 1在盐胁迫下的功能。我们发现GmCLC 1基因增强了转基因拟南芥的耐盐性。盐胁迫对植物生长的影响主要是通过减少地上部Cl-的积累来缓解的。在大豆毛状根中过量表达GmCLC 1可将更多的Cl-截留在根中，而将更少的Cl-转移到芽中，从而导致根和叶中的相对电解质渗漏值降低。将大豆GmCLC 1或酵母氯离子转运蛋白基因GEF 1转化到酵母gef 1突变体中，用不同的氯化物盐（MnCl 2、KCl、NaCl）处理，可以提高酵母的存活率。结果表明，GmCLC 1和GEF 1在缓解酵母gef 1突变株不同氯盐胁迫方面具有相似的效果。总之，这项工作表明，在Cl-胁迫下GmCLC 1的保护功能。
The family of chloride channel proteins that mediate Cl- transportation play vital roles in plant nutrient supply, cellular action potential and turgor pressure adjustment, stomatal movement, hormone signal recognition and transduction, Cl- homeostasis, and abiotic and biotic stress tolerance. The anionic toxicity, mainly caused by chloride ions (Cl-), on plants under salt stress remains poorly understood. In this work, we investigated the function of soybean Cl-/H+ antiporter GmCLC1 under salt stress in transgenic Arabidopsis thaliana, soybean, and yeast. We found that GmCLC1 enhanced salt tolerance in transgenic A. thaliana by reducing the Cl- accumulation in shoots and hence released the negative impact of salt stress on plant growth. Overexpression of GmCLC1 in the hairy roots of soybean sequestered more Cl- in their roots and transferred less Cl- to their shoots, leading to lower relative electrolyte leakage values in the roots and leaves. When either the soybean GmCLC1 or the yeast chloride transporter gene, GEF1, was transformed into the yeast gef1 mutant, and then treated with different chloride salts (MnCl2, KCl, NaCl), enhanced survival rate was observed. The result indicates that GmCLC1 and GEF1 exerted similar effects on alleviating the stress of diverse chloride salts on the yeast gef1 mutant. Together, this work suggests a protective function of GmCLC1 under Cl- stress.