Transcriptome Analysis of Ovarian Follicles Reveals Potential Pivotal Genes Associated With Increased and Decreased Rates of Chicken Egg Production.
Transcriptome Analysis of Ovarian Follicles Reveals Potential Pivotal Genes Associated With Increased and Decreased Rates of Chicken Egg Production.
复制标题
卵巢卵泡的转录组分析揭示了与鸡蛋产量增加和减少相关的潜在关键基因
DOI：
10.3389/fgene.2021.622751
复制
发表时间：
2021
影响因子：
3.7
通讯作者：
Gonzalez JM
中科院分区：
文献类型：
--
作者：
Chen X;Sun X;Chimbaka IM;Qin N;Xu X;Liswaniso S;Xu R;Gonzalez JM
蛋生产是商品家禽业的一项重要经济性状。卵泡发育在蛋鸡生产性能和生殖生理调节中起着关键作用。然而，蛋鸡卵泡发育的各个阶段所涉及的关键基因和信号通路仍然知之甚少。本研究采用rna测序方法，对比分析了高（HR）和低（LR）产蛋率母鸡三个发育阶段的卵泡，即大白卵泡（LWF）、小黄卵泡（SYF）和大黄卵泡（LYF）的转录组。构建了18个cDNA文库，分别有236、544和386个单基因在HR和LR母鸡的LWF、SYF和LYF卵泡中表达显著差异。其中LWF和SYF共转录差异表达基因（DEGs） 47个，SYF和LYF共表达DEGs 68个，LWF和LYF共表达DEGs 54个。在LWF、SYF和LYF卵泡中发现了13个共表达的deg。18个候选基因，包括P2RX1、CAB39L、BLK、CSMD3、GPR65、ADRB2、CSMD1、PLPP4、ATF3、PRLL、STMN3、RORB、PIK3R1、PERP1、ACSBG1、MRTO4、CDKN1A和EDA2R，被鉴定出可能与产蛋有关。此外，京都基因和基因组百科分析表明，神经活性配体-受体相互作用、细胞粘附分子、过氧化物酶体增殖体激活受体途径和cAMP信号通路可能通过影响卵泡发育而在卵泡产蛋性状的形成中发挥重要作用。本研究首次对HR和LR母鸡不同大小卵泡进行转录组分析。这些结果为阐明鸡卵泡发育与产蛋相关的遗传机制提供了有用的分子证据。
Egg production is an important economic trait in the commercial poultry industry. Ovarian follicle development plays a pivotal role in regulation of laying hen performance and reproductive physiology. However, the key genes and signaling pathways involved in the various-stages of laying hen follicular development remain poorly understood. In this study, transcriptomes of ovarian follicles at three developmental stages, the large white follicle (LWF), small yellow follicle (SYF), and large yellow follicle (LYF), were comparatively analyzed in hens with high (HR) and low (LR) egg-laying rates by RNA-sequencing. Eighteen cDNA libraries were constructed and a total of 236, 544, and 386 unigenes were significantly differentially expressed in the LWF, SYF, and LYF follicles of HR and LR hens, respectively. Among them, 47 co-transcribed differentially expressed genes (DEGs) in LWF and SYF, 68 co-expressed DEGs in SYF and LYF, and 54 co-expressed DEGs in LWF and LYF were mined. Thirteen co-expressed DEGs were found in LWF, SYF, and LYF follicles. Eighteen candidate genes, including P2RX1, CAB39L, BLK, CSMD3, GPR65, ADRB2, CSMD1, PLPP4, ATF3, PRLL, STMN3, RORB, PIK3R1, PERP1, ACSBG1, MRTO4, CDKN1A, and EDA2R were identified to be potentially related to egg production. Furthermore, Kyoto Encyclopedia of Genes and Genomes analysis indicated neuroactive ligand-receptor interaction, cell adhesion molecules, peroxisome proliferator-activated receptor pathway, and cAMP signaling pathway might elicit an important role in formation of egg-laying traits by influencing ovarian follicle development. This study represents the first transcriptome analysis of various-sized follicles between HR and LR hens. These results provide useful molecular evidence for elucidating the genetic mechanism underlying ovarian follicle development associated with egg production in chicken.
登录
查看更多内容
影响因子：
2.8
作者：
Chen, L. -R.;Lee, S. -C.;Shiue, Y. -L.
通讯作者：
Shiue, Y. -L.
影响因子：
4.4
作者：
Kang, B.;Guo, J. R.;Dong, C. Y.
通讯作者：
Dong, C. Y.
影响因子：
7
作者：
Court, Franck;Le Boiteux, Elisa;Arnaud, Philippe
通讯作者：
Arnaud, Philippe
影响因子：
0.7
作者：
Dupont, Joelle;Reverchon, Maxime;Rame, Christelle
通讯作者：
Rame, Christelle
影响因子：
5.4
作者：
Kaese, Miriam;Galuska, Christina E.;Galuska, Sebastian P.
通讯作者：
Galuska, Sebastian P.