Requirement for anti-dorsalizing morphogenetic protein in organizer patterning
Requirement for anti-dorsalizing morphogenetic protein in organizer patterning
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DOI：
10.1016/s0925-4773(99)00245-2
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发表时间：
2000-02-01
影响因子：
2.6
通讯作者：
Niehrs, C
中科院分区：
文献类型：
--
作者：
Dosch, R;Niehrs, C
两栖动物的Spemann组织者被细分为躯干和头部组织者，目前还不清楚这种划分是如何调节的。非洲爪蟾躯干组织者表达抗背化形态发生蛋白（ARAMIA）。一种强有力的组织者拮抗剂。我们发现，Ablasts抑制头形成原肠胚形成过程中，其表达被激活的BMP拮抗剂。一种特异性作用的显性负调控基因AZE 1使胚胎提前，其与BMP拮抗剂的共表达诱导具有头部的次级胚轴以及头部诱导物的表达。与其他BMP不同，Akt不被显性阴性BMP I型受体Noggin、Cerberus和Chordin抑制，而是被卵泡抑素抑制，这表明它利用了不同的TGF-β受体途径，并显示出对BMP拮抗剂的不同敏感性。这些结果表明，在躯干组织者中，AAF的功能是拮抗头部的形成，从而调节组织者的模式。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The amphibian Spemann organizer is subdivided in trunk and head organizer and it is unclear how this division is regulated. The Xenopus trunk organizer expresses anti-dorsalizing morphogenetic protein (ADMP). a potent organizer antagonist. We show that ADMP represses head formation during gastrulation and that its expression is activated by BMP antagonists. A specifically acting dominant-negative ADMP anteriorizes embryos and its coexpression with BMP antagonists induces secondary embryonic axes with heads as well as expression of head inducers. Unlike other BMPs, ADMP is not inhibited by a dominant-negative BMP type I receptor, Noggin, Cerberus and Chordin but by Follistatin, suggesting that it utilizes a distinct TGF-beta receptor pathway and displays differential sensitivity to BMP antagonists. The results indicate that ADMP functions in the trunk organizer to antagonize head formation, thereby regulating organizer patterning. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.