Synergistic effects of antigen and soluble T-cell factors in B-lymphocyte activation.
Synergistic effects of antigen and soluble T-cell factors in B-lymphocyte activation.
复制标题
抗原和可溶性 T 细胞因子在 B 淋巴细胞激活中的协同作用。
DOI：
--
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
S. Pierce
中科院分区：
文献类型：
--
作者：
M. L. Jelachich;M. Grusby;D. Clark;D. Tasch;E. Margoliash;S. Pierce
小鼠EL4胸腺瘤或几个小鼠T细胞杂交瘤细胞经其特异性抗原或刀豆蛋白A刺激后，经佛波酯12-肉豆蔻酸酯13-醋酸酯激活的培养上清，可诱导原代脾B细胞增殖并分化为抗体分泌细胞。这种作用不是由于白介素2，也不需要巨噬细胞的存在。抗体反应是多克隆的，包括针对2，4-二硝基苯基和鸽子细胞色素c的抗体，其数量占产生的总免疫球蛋白的1%或更少。加入这两种抗原中的任何一种都会增加相应的特异性抗体的数量。当二硝基苯基血蓝蛋白浓度很高时，这种特异性反应可能会受到抑制。这些观察表明，可溶性T细胞因子足以激活一部分幼稚B细胞分泌抗体，而在体外这些条件下，抗原的存在只是增强了特异性反应。
Supernatants from phorbol 12-myristate 13-acetate-activated cultures of the mouse EL4 thymoma, or of several mouse T-cell hybridomas stimulated either by their specific antigen or by concanavalin A, induced primary splenic B cells to proliferate and differentiate to antibody-secreting cells. This effect was not due to interleukin 2 and did not require the presence of macrophages. The antibody response was polyclonal, including antibodies specific for 2,4-dinitrophenyl and pigeon cytochrome c, present in amounts of 1% or less of the total immunoglobulin produced. The addition of either of these antigens increased the amount of the corresponding specific antibody. At very high concentrations of dinitrophenyl-hemocyanin the specific response could be depressed. These observations were taken to demonstrate that soluble T-cell factors are sufficient to activate a portion of naive B cells to antibody secretion and that under these conditions in vitro the presence of antigen merely enhances the specific response.