Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia coli Colonization Factors and Toxins
Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia coli Colonization Factors and Toxins
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DOI：
10.1128/jcm.00316-09
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发表时间：
2009-04-01
影响因子：
9.4
通讯作者：
Sjoeling, Asa
中科院分区：
文献类型：
--
作者：
Rodas, Claudia;Iniguez, Volga;Sjoeling, Asa
开发了4种用于检测19种产肠毒素大肠杆菌（ETEC）定植因子的多重PCR方法和一种改进的ETEC毒素多重PCR方法，并在孟加拉国和玻利维亚的ETEC菌株保藏中进行了测试。该检测试剂盒可用于监测可使用PCR的诊断实验室中的ETEC感染。
Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.