DIMINISHED INTERNALIZATION AND ACTION OF 1,25-DIHYDROXYVITAMIN D3 IN DERMAL FIBROBLASTS CULTURED FROM NEW WORLD PRIMATES
DIMINISHED INTERNALIZATION AND ACTION OF 1,25-DIHYDROXYVITAMIN D3 IN DERMAL FIBROBLASTS CULTURED FROM NEW WORLD PRIMATES
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DOI：
10.1210/endo-116-6-2523
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发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
RUDE, RK
中科院分区：
文献类型：
--
作者：
ADAMS, JS;GACAD, MA;RUDE, RK
在洛杉矶动物园的新世界灵长类动物群Saguinus imperator中研究了1，25-二羟维生素D3 [1，25-（OH）2D 3]抗性骨软化症的发生。与旧世界灵长类动物相比，新世界灵长类动物中1，25-（OH）2D 3的平均血清浓度升高了5倍。新世界灵长类动物培养的皮肤成纤维细胞对0.6 nM [3 H] 1，25-（OH）2D 3的特异性内化，与旧世界灵长类动物或人的细胞相比减少了75%。（OH）2D 3诱导的25-羟维生素-D3 -24-羟化酶活性降低90-95%。与旧世界灵长类动物的血清相比，新世界灵长类动物血清对1，25-（OH）2D 3的能力或亲和力没有改变。显然，新世界灵长类动物中维生素D抵抗性骨软化症的发生是靶细胞对1，25-（OH）2D 3的高亲和力受体介导摄取降低的结果。
The occurrence of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]-resistant osteomalacia was investigated in the New World primate colony of Saguinus imperator at the Los Angeles Zoo. The mean serum concentration of 1,25-(OH)2D3 was elevated 5-fold in the New World primates compared to that in their Old World counterparts. The specific internalization of 0.6 nM [3H]1,25-(OH)2D3 by cultured dermal fibroblasts from New World primates was reduced 75% compared to that by cells from Old World primates or man. The decrease in hormone uptake resulted from a decrease in the number of high affinity intracellular binding sites for 1,25-(OH2D3, and it apparently caused a 90-95% reduction in 1,25-(OH)2D3-induced 25-hydroxyvitamin-D3-24-hydroxylase activity. There was no alteration in the capacity or avidity of New World primate serum for 1,25-(OH)2D3 compared to that of serum from Old World primates. Apparently, the occurrence of vitamin D-resistant osteomalacia in New World primates is the result of decreased high affinity, receptor-mediated uptake of 1,25-(OH)2D3 by the target cell.