The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats
The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats
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DOI：
10.1007/s00223-016-0156-0
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发表时间：
2016-10-01
影响因子：
4.2
通讯作者：
Sehmisch, S.
中科院分区：
文献类型：
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作者：
Komrakova, M.;Hoffmann, D. B.;Sehmisch, S.
本研究的目的是研究特立帕肽（PTH）或雷奈酸锶（SR）与全身振动（WBV）联合治疗对骨质减少大鼠模型中骨愈合和肌肉特性的影响。对72只大鼠（3月龄）进行双侧卵巢切除（Ovx），12只大鼠保持完整（Non-Ovx）。8周后，在所有大鼠胫骨干骺端进行双侧横向截骨。之后，将Ovx大鼠分为6组（n = 12）：（1）Ovx-无治疗组，（2）Ovx +振动（Vib）组，（3）SR组，（4）SR + Vib组，（5）PTH组，和（6）PTH + Vib组。在卵巢切除术当天应用PTH（40 μ g/kg BW sc.5x/周）和SR（613 mg/kg BW，每日食物中），5天后振动处理（垂直，70 Hz，0.5 mm，2x/天，持续15 min）长达6周。在WBV + SR组中，与单独SR相比，骨痂密度、骨小梁数量以及Alp和Oc基因表达降低。在WBV + PTH组中，皮质和骨痂宽度、生物力学特性、Opg基因表达和Opg/Rankl比率增加;与单独PTH相比，皮质和骨痂密度降低。在两个振动组中均发现一例非桥接。单独振动并没有改变骨参数; PTH具有比SR治疗更强的效果。在肌肉中，联合治疗改善了Ovx大鼠的纤维大小。WBV可单独应用或与抗骨质疏松药物治疗联合应用，以改善肌肉组织。然而，在骨折患者中，抗骨质疏松治疗和振动的应用可能对骨愈合产生不利影响。
The aim of the present study was to study the effect of combined therapy of teriparatide (PTH) or strontium ranelate (SR) with whole-body vibration (WBV) on bone healing and muscle properties in an osteopenic rat model. Seventy-two rats (3 months old) were bilaterally ovariectomized (Ovx), and 12 rats were left intact (Non-Ovx). After 8 weeks, bilateral transverse osteotomy was performed at the tibia metaphysis in all rats. Thereafter, Ovx rats were divided into six groups (n = 12): (1) Ovx-no treatment, (2) Ovx + vibration (Vib), (3) SR, (4) SR + Vib, (5) PTH, and (6) PTH + Vib. PTH (40 mu g/kg BW sc. 5x/week) and SR (613 mg/kg BW in food daily) were applied on the day of ovariectomy, vibration treatments 5 days later (vertical, 70 Hz, 0.5 mm, 2x/day for 15 min) for up to 6 weeks. In the WBV + SR group, the callus density, trabecular number, and Alp and Oc gene expression were decreased compared to SR alone. In the WBV + PTH group, the cortical and callus widths, biomechanical properties, Opg gene expression, and Opg/Rankl ratio were increased; the cortical and callus densities were decreased compared to PTH alone. A case of non-bridging was found in both vibrated groups. Vibration alone did not change the bone parameters; PTH possessed a stronger effect than SR therapy. In muscles, combined therapies improved the fiber size of Ovx rats. WBV could be applied alone or in combination with anti-osteoporosis drug therapy to improve muscle tissue. However, in patients with fractures, anti-osteoporosis treatments and the application of vibration could have an adverse effect on bone healing.