Nucleotide sequence of pOLA52:: A conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux
Nucleotide sequence of pOLA52:: A conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux
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DOI：
10.1016/j.plasmid.2008.03.003
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发表时间：
2008-07-01
期刊：
影响因子：
2.6
通讯作者：
Sorensen, Soren Johannes
中科院分区：
文献类型：
--
作者：
Norman, Anders;Hansen, Lars Hestbjerg;Sorensen, Soren Johannes
对大的接合性多药耐药（MDR）质粒pOLA 52进行测序和注释。该质粒编码两种通常与机会致病菌染色体相关的表型，即通过耐药-增殖-分裂（RND）型外排泵（oqxAB）的MDR和3型菌毛（mrkABCDF）的形成。该质粒长51，602 bp，含有68个推定基因。大约一半的质粒构成了一个保守的IncX 1型骨架与预测的区域接合，复制和分区，以及毒素/抗毒素（TA）质粒成瘾系统。通过不相容性试验，该质粒也被归类为IncX 1。因此，pOLA 52的接合转移和质粒维持区似乎代表了充分表征的IncX 2质粒R6 K的IncX 1直向同源物。GenBank中的序列同源性搜索也表明IncX 1组质粒的流行率比IncX 2高得多。pOLA 52的21 kb“遗传负荷”区域显示由嵌合体组成，其中包括编码氨苄青霉素抗性的片段化Tn 3转座子。最值得注意的是，oqxAB和mrkABCDF盒包含在似乎源自肺炎克雷伯氏菌的两个复合转座子（Tn 6010和Tn 6011）内，从而证明了IncX 1质粒促进基因盒在不同肠杆菌科之间横向转移的能力。(C)2008年爱思唯尔公司All rights reserved.
The large conjugative multidrug resistance (MDR) plasmid pOLA52 was sequenced and annotated. The plasmid encodes two phenotypes normally associated with the chromosomes of opportunistic pathogens, namely MDR via a resistance-nodulation-division (RND)-type efflux-pump (oqxAB), and the formation of type 3 fimbriae (mrkABCDF). The plasmid was found to be 51,602 bp long with 68 putative genes. About half of the plasmid constituted a conserved IncX1-type backbone with predicted regions for conjugation, replication and partitioning, as well as a toxin/antitoxin (TA) plasmid addiction system. The plasmid was also classified as IncX1 with incompatibility testing. The conjugal transfer and plasmid maintenance regions of pOLA52 therefore seem to represent IncX1 orthologues of the well-characterized IncX2 plasmid R6K. Sequence homology searches in GenBank also suggested a considerably higher prevalence of IncX1 group plasmids than IncX2. The 21 kb 'genetic load' region of pOLA52 was shown to consist of a mosaic, among other things a fragmented Tn3 transposon encoding ampicillin resistance. Most notably the oqxAB and mrkABCDF cassettes were contained within two composite transposons (Tn6010 and Tn6011) that seemed to originate from Klebsiella pneumoniae, thus demonstrating the capability of IncX1 plasmids of facilitating lateral transfer of gene cassettes between different Enterobacteriaceae. (C) 2008 Elsevier Inc. All rights reserved.