Extensive sequence homology at the 3'-termini of the four RNAs of cucumber mosaic virus.
Extensive sequence homology at the 3'-termini of the four RNAs of cucumber mosaic virus.
复制标题
黄瓜花叶病毒的四种 RNA 的 3 末端具有广泛的序列同源性。
DOI：
10.1093/nar/7.4.825
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发表时间：
1979
影响因子：
14.9
通讯作者：
R. Symons
中科院分区：
文献类型：
--
作者：
R. Symons
以d（pT 8 G）为引物，以2 ′，3 ′-双脱氧核苷5 ′-三磷酸为特异性链终止剂，用逆转录酶体外复制了黄瓜花叶病毒4种RNA的3 ′-末端270个残基的序列。RNA 3和4的末端序列是相同的;这是预期的，因为杂交数据已经显示RNA 4的序列存在于RNA 3的3 '末端（Gould和Symons（1978）Eur. 91，269-278）。RNA 1和2的前138个残基与RNA 3和4的前138个残基相同，除了RNA 1中的一个残基和RNA 2中的三个残基。从3 ′-末端的残基139到270，RNA 1和2显示，相对于RNA 3和4，33个残基的非同源区，40个残基的同源区，14个残基的部分同源区，其可能延伸到约300个残基。RNA 1和RNA 2之间有11个残基不同。
The sequences of 270 residues from the 3'-termini of the four RNAs of cucumber mosaic virus have been determined by copying the in vitro polyadenylated RNAs with reverse transcriptase using d(pT8G) as primer and the 2',3'-dideoxynucleoside 5'-triphosphates as specific chain terminators. The terminal sequences of RNAs 3 and 4 were identical; this was expected since hybridization data has shown that the sequence of RNA 4 was present at the 3'-end of RNA 3 (Gould and Symons (1978) Eur. J. Biochem. 91, 269-278). The first 138 residues of RNAs 1 and 2 were identical to those of RNAs 3 and 4 except for one residue in RNA 1 and three residues in RNA 2. From residue 139 to 270 from the 3'-terminus, RNAs 1 and 2 showed, relative to RNAs 3 and 4, a non-homologous region of 33 residues, a homologous region of 40 residues, a partially homologous region of 14 residues which probably extended to about residue 300. There were 11 residues different between RNAs 1 and 2.