ACTIN GENE FAMILY OF CAENORHABDITIS-ELEGANS
ACTIN GENE FAMILY OF CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0022-2836(83)90056-6
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
HIRSH, D
中科院分区：
文献类型：
--
作者：
FILES, JG;CARR, S;HIRSH, D
从秀丽隐杆线虫中分离出四个肌动蛋白基因，这些基因解释了所有主要肌动蛋白杂交与总基因组DNA。肌动蛋白基因I，II和III被聚集在12时。 103基地区域； Gene IV与其他人无关。将所有4个基因从至少核苷酸-109至+250进行测序。对于第一个307个编码核苷酸，基因I和III是相同的。基因I和II在前250个编码核苷酸中的14个位置有所不同。一种差异将ASP取代为CORDON 5的GLU。基因I和IV在前259个编码核苷酸中的18个位置有所不同，而不会引起任何氨基酸差异。基因I，II和III具有密码子64的第一个核苷酸和Gene IV的核苷酸后具有内含子，并在密码子19和20之间具有内含子。到目前为止，4个核苷酸序列定义了2个不同的氨基酸序列。这两个氨基酸序列都比脊椎动物肌动蛋白更类似于脊椎动物细胞质肌动蛋白。 Bristol和Bergerac菌株之间的DNA多态性被用作遗传杂交中的表型标记物，以在UNC-23和SMA-1之间的链接组V中绘制肌动蛋白基因的簇，以开始分子遗传肌动蛋白基因座的分析。
Four actin genes were isolated from C. elegans that account for all of the major actin hybridization to total genomic DNA. Actin genes I, II and III are clustered within a 12 .times. 103 base region; gene IV is unlinked to the others. All 4 genes were sequenced from at least nucleotide -109 to +250. Genes I and III are identical for the first 307 coding nucleotides. Genes I and II differ in 14 positions within the first 250 coding nucleotides; one difference substitutes an Asp for a Glu at codon 5. Genes I and IV differ in 18 positions within the first 259 coding nucleotides without causing any amino acid differences. Genes I, II and III have introns after the first nucleotide of codon 64 and gene IV has an intron between codons 19 and 20. The 4 nucleotide sequences thus far define 2 different amino acid sequences. Both of the amino acid sequences resemble vertebrate cytoplasmic actin more than vertebrate muscle actin. A DNA polymorphism between the Bristol and Bergerac strains was used as a phenotypic marker in genetic crosses to map the cluster of actin genes within a 2% recombination interval on linkage group V between unc-23 and sma-1 in order to begin a molecular genetic analysis of the actin loci.