The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants
The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants
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DOI：
10.1093/jexbot/52.356.541
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发表时间：
2001-04-01
影响因子：
6.9
通讯作者：
Wells, DM
中科院分区：
文献类型：
--
作者：
Miller, AJ;Cookson, SJ;Wells, DM
微电极测量可用于研究单个活细胞的细胞内离子池和膜运输过程。微电极可在完整植物的根和叶细胞的表面层中记录这些过程。通过仔细操作植物，产生最小的破坏，因此从这些测量中获得的信息最有可能代表“体内”情况。微电极可用于测定细胞质膜中特定转运系统的活性。无机代谢物离子的房室浓度已用几种不同的方法测定，并对胞质溶胶所得结果进行了比较。离子选择性微电极已用于测定单细胞质外体、胞质溶胶和液泡中离子的活性。正在生产用于这些微电极的新传感器，其提供较低的检测限和测量其他先前未测量的离子的机会，测量可用于确定细胞内稳态活动或报告细胞对环境变化的响应。
Microelectrode measurements can be used to investigate both the intracellular pools of ions and membrane transport processes of single living cells, Microelectrodes can report these processes in the surface layers of root and leaf cells of intact plants. By careful manipulation of the plant, a minimum of disruption is produced and therefore the information obtained from these measurements most probably represents the 'in vivo' situation. Microelectrodes can be used to assay for the activity of particular transport systems in the plasma membrane of cells, Compartmental concentrations of inorganic metabolite ions have been measured by several different methods and the results obtained for the cytosol are compared, ion-selective microelectrodes have been used to measure the activities of ions in the apoplast, cytosol and vacuole of single cells. New sensors for these microelectrodes are being produced which offer lower detection limits and the opportunity to measure other previously unmeasured ions, Measurements can be used to determine the intracellular steady-state activities or report the response of cells to environmental changes.