Bioorthogonal human milk oligosaccharide probes for antimicrobial target identification within Streptococcus agalactiae
Bioorthogonal human milk oligosaccharide probes for antimicrobial target identification within Streptococcus agalactiae
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DOI：
10.1016/j.carres.2019.107895
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发表时间：
2020-02-01
影响因子：
3.1
通讯作者：
Townsend, Steven D.
中科院分区：
文献类型：
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作者：
Chambers, Schuyler A.;Townsend, Steven D.
人乳低聚糖（HMO）是一类结构多样的碳水化合物，对无乳链球菌（B族链球菌，GBS）具有较强的抗菌活性。这项工作突出了GBS中几个HMO生物正交探针的设计，合成和保留的生物活性，这是一流的进步。这种探针的使用将有助于确定GBS内的HMO-蛋白质相互作用，并可广泛应用于研究各种生物系统内的HMO细胞靶点。最后，这种策略非常适合其他寡糖支架，需要最小的合成转化和化学扰动。
Human milk oligosaccharides (HMOs) are a structurally diverse class of carbohydrates that possess strong antibacterial activity against Streptococcus agalactiae (Group B Strep, GBS). This work highlights the design, synthesis, and retained biological activity of several HMO bioorthogonal probes within GBS, a first in class advance. The use of such probes will assist in identifying HMO-protein interactions within GBS and may be broadly applicable in researching HMO cellular targets within a variety of biological systems. Finally, this strategy is highly amenable to other oligosaccharide scaffolds, requiring minimal synthetic transformations and chemical perturbation.