Miscibility and in vitro osteocompatibility of biodegradable blends of poly[(ethyl alanato) (p-phenyl phenoxy) phosphazene] and poly(lactic acid-glycolic acid)
Miscibility and in vitro osteocompatibility of biodegradable blends of poly[(ethyl alanato) (p-phenyl phenoxy) phosphazene] and poly(lactic acid-glycolic acid)
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DOI：
10.1016/j.biomaterials.2007.09.029
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发表时间：
2008-01-01
期刊：
影响因子：
14
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
--
作者：
Deng, Meng;Nair, Lakshmi S.;Laurencin, Cato T.
以前，我们证明了乙基甘氨酸取代的聚磷腈的能力，以中和酸性降解产物和控制降解速率的聚（乳酸-乙醇酸）（PLAGA）的混合。在这项研究中，使用互溶剂方法制备了高强度聚[（50%乙基丙氨酰）（50%对苯基苯氧基）磷腈]（PNEA（50）PhPh（50））和85：15 PLAGA的共混物。研究了二氯甲烷（MC）、氯仿（CF）和四氢呋喃（THF）三种不同溶剂对共混物相容性的影响。然后使用THF作为互溶剂，以不同的重量比制备三种不同的混合物，即25：75（BLEND 25）、50：50（BLEND 50）和75：25（BLEND 75）。通过扫描电镜（SEM）、差示扫描量热法（DSC）和傅里叶变换红外光谱（FTIR）对共混物的相容性进行了评价。其中，BLEND 25是混溶的，而BLEND 50和BLEND 75是部分混溶的。此外，BLEND 25在其表面上形成磷灰石层，如在所进行的仿生研究中所证明的。这些新的共混物显示出与PLAGA相当的细胞粘附和增殖。然而，混合物中的PNEA（50）PhPh（50）组分能够增加体外原代大鼠成骨细胞（PRO）的表型表达和矿化基质合成。高强度PNEA（50）PhPh（50）和85：15 PLAGA的混合物是用于各种肌肉骨骼应用的有前途的生物材料。(C)2007爱思唯尔有限公司保留所有权利。
Previously we demonstrated the ability of ethyl glycinato substituted polyphosphazenes to neutralize the acidic degradation products and control the degradation rate of poly(lactic acid-glycolic acid) (PLAGA) by blending. In this study, blends of high strength poly [(50% ethyl alanato) (50% p-phenyl phenoxy) phosphazene] (PNEA(50)PhPh(50)) and 85:15 PLAGA were prepared using a mutual solvent approach.. Three different solvents, methylene chloride (MC), chloroform (CF) and tetrahydrofuran (THF) were studied to investigate solvent effects on blend miscibility. Three different blends were then fabricated at various weight ratios namely 25:75 (BLEND25), 50:50 (BLEND50), and 75:25 (BLEND75) using THF as the mutual solvent. The miscibility of the blends was evaluated by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). Among these, BLEND25 was miscible while BLEND50 and BLEND75 were partially miscible. Furthermore, BLEND25 formed apatite layers on its surface as evidenced in a biomimetic study performed. These novel blends showed cell adhesion and proliferation comparable to PLAGA. However, the PNEA(50)PhPh(50) component in the blends was able to increase the phenotypic expression and mineralized matrix synthesis of the primary rat osteoblasts (PRO) in vitro. Blends of high strength PNEA(50)PhPh(50) and 85:15 PLAGA are promising biomaterials for a variety of musculoskeletal applications. (C) 2007 Elsevier Ltd. All rights reserved.