Genetic functions and cell-cell interactions in the pheromone-inducible plasmid transfer system of Enterococcus faecalis.
Genetic functions and cell-cell interactions in the pheromone-inducible plasmid transfer system of Enterococcus faecalis.
复制标题
粪肠球菌信息素诱导质粒转移系统中的遗传功能和细胞间相互作用。
DOI：
10.1111/j.1365-2958.1990.tb00639.x
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发表时间：
1990
影响因子：
3.6
通讯作者：
Dunny,GM
中科院分区：
文献类型：
--
作者：
Dunny,GM
信息素诱导的质粒转移是一种新的细菌接合形式，迄今为止仅在粪肠球菌（链球菌）中观察到。该过程包括供体和受体细胞之间相互作用的几个重要阶段。最初的相互作用是将化学信号从受体传递到供体细胞。最近的证据表明，该信号是以小的疏水肽的形式，其能够在低至1-5个分子/应答细胞的浓度下在供体细胞中诱导复杂的交配应答。大多数。粪肠杆菌菌株产生多种信息素，每种信息素仅在携带特定质粒（或相关质粒家族的成员）的细胞中诱导应答。归因于信息素反应的遗传功能包括：（i）细胞-细胞聚集，其促进交配细胞之间的初始紧密接触;（ii）表面排斥，其防止聚集的供体细胞之间的质粒转移;和（iii）高效的DNA转移，其除了聚集之外还需要其他未鉴定的功能。前两个过程似乎由蛋白质表面抗原介导。
Pheromone‐inducible plasmid transfer is a novel form of bacterial conjugation which has, to date, been observed only inEnterococcus (Streptococcus) faecalis.This process includes several important stages of interaction between the donor and recipient cell. The initial interaction is the transmission of a chemical signal from the recipient to the donor cell. Recent evidence has shown that the signal is in the form of a small hydrophobic peptide, which is capable of inducing a complex mating response in the donor cell at concentrations as low as 1–5 molecules per responder cell. MostE. faecalisstrains produce multiple pheromones, each of which induces a response only in cells carrying a particular plasmid (or member of a family of related plasmids). Genetic functions ascribed to the pheromone response include: (i) cell‐cell aggregation, which promotes initial close contact between mating cells; (ii) surface exclusion, which prevents plasmid transfer between aggregated donor cells; and (iii) highly efficient DNA transfer, which requires other unidentified functions in addition to aggregation. The first two processes appear to be mediated by proteinaceous surface antigens.