Interspecific symbiont transfection confers a novel ecological trait to the recipient insect
Interspecific symbiont transfection confers a novel ecological trait to the recipient insect
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DOI：
10.1098/rsbl.2010.0699
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发表时间：
2011-04-23
期刊：
影响因子：
3.3
通讯作者：
Fukatsu, Takema
中科院分区：
文献类型：
--
作者：
Tsuchida, Tsutomu;Koga, Ryuichi;Fukatsu, Takema
在日本，豌豆蚜主要以野豌豆和三叶草为食，许多蚜无性系在野豌豆上的后代比在三叶草上的后代多。在这种情况下，特定的基因型的兼性共生体Regiella insecticola增强繁殖感染的豌豆蚜虫，特别是在三叶草，从而扩大了合适的食物植物范围的昆虫。一个与A.豌豆、野豌豆蚜虫粗尾野豌豆蚜虫通常在野豌豆上发现，但在三叶草上没有。实验室饲养的M. crassicauda菌株在野豌豆上表现出活跃的繁殖，但在三叶草上基本上没有繁殖。实验转染A. pisum为M.通过血淋巴注射粗尾丝虫在受体中建立了稳定的和可遗传的感染，尽管在自然群体中没有检测到Regiella感染。粗尾类不同菌株的Regiella转染M.粗尾蚜在箭舌豌豆上生长繁殖，但与相应的未感染蚜虫品系相比，其适合度较低。引人注目的是，Regiella转染的M. crassicauda在三叶草上表现出提高的存活率和一些繁殖。这些结果表明，Regiella具有赋予新的昆虫宿主一种生态学特性的潜力，即对三叶草的适应，也解释了为什么Regiella能够感染M. crassicauda，但很少发现在这些蚜虫种群。
In Japan, pea aphids Acyrthosiphon pisum mainly feed on vetch and clover, and many aphid clones produce more progeny on vetch than on clover. In this context, particular genotypes of the facultative symbiont Regiella insecticola enhance reproduction of infected pea aphids specifically on clover, thereby broadening the suitable food plant range of the insect. A species that is sympatric to A. pisum, vetch aphids Megoura crassicauda, are commonly found on vetch but not on clover. Laboratory rearing of M. crassicauda strains revealed active reproduction on vetch but substantially no reproduction on clover. Experimental transfection of Regiella from A. pisum to M. crassicauda by haemolymph injection established stable and heritable infection in the recipients, although no Regiella infection has been detected in natural populations of M. crassicauda. Different strains of Regiella-transfected M. crassicauda grew and reproduced on vetch, but exhibited lower fitness in comparison with corresponding uninfected aphid strains. Strikingly, the Regiella-transfected M. crassicauda exhibited improved survival and some reproduction on clover. These results suggest that Regiella has the potential to confer an ecological trait, adaptation to clover, on novel insect hosts, and also account for why Regiella is able to infect M. crassicauda but is scarcely found in these aphid populations.