Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin.
Genomic Comparison of the Closely Related Salmonella enterica Serovars Enteritidis and Dublin.
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DOI：
10.2174/1874285801206010005
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Chabalgoity JA
中科院分区：
文献类型：
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作者：
Betancor L;Yim L;Martínez A;Fookes M;Sasias S;Schelotto F;Thomson N;Maskell D;Chabalgoity JA
肠炎沙门氏菌与都柏林血清型亲缘关系密切，但致病性和流行病学差异显著。肠炎沙门氏菌是一种宿主范围广泛的血清型，通常引起肠胃炎，很少引起人类的侵袭性疾病。都柏林沙门氏菌主要寄生于牛，但一旦感染人类，通常会导致侵袭性疾病。为了更广泛地了解这些差异的程度，我们对来自每个血清型的几个田间分离株进行了基于微阵列的比较基因组学研究。在沙门氏菌中，基因组降解与宿主适应相关，因此我们还在全基因组尺度上比较了它们的可用基因组序列，以评估每个血清型中的假基因组成。微阵列分析显示两个血清型共有3771个CDS，其中33个仅存在于肠炎，87个为都柏林独有。假基因鉴定结果显示，都柏林沙门氏菌中有177个失活CDS与肠炎沙门氏菌的活性基因相对应，其中9个在宿主适应的鸡产沙门氏菌和霍乱沙门氏菌血清型中也无活性。对多个S. Dublin临床分离株的这9个CDS进行测序，结果显示它们都是假基因，这表明这一特征不是所测序菌株所特有的。在这些CDS中，shdA （Peyer’s patch定植因子）和mglA（半乳糖苷运输ATP结合蛋白）在人类适应型伤寒沙门氏菌和副伤寒沙门氏菌中似乎也不活跃，这表明这些基因的功能可能与某些沙门氏菌血清型感染广泛宿主的能力有关。
The Enteritidis and Dublin serovars of Salmonella enterica are closely related, yet they differ significantly in pathogenicity and epidemiology. S. Enteritidis is a broad host range serovar that commonly causes gastroenteritis and infrequently causes invasive disease in humans. S. Dublin mainly colonizes cattle but upon infecting humans often results in invasive disease.To gain a broader view of the extent of these differences we conducted microarray-based comparative genomics between several field isolates from each serovar. Genome degradation has been correlated with host adaptation in Salmonella, thus we also compared at whole genome scale the available genomic sequences of them to evaluate pseudogene composition within each serovar. Microarray analysis revealed 3771 CDS shared by both serovars while 33 were only present in Enteritidis and 87 were exclusive to Dublin. Pseudogene evaluation showed 177 inactive CDS in S. Dublin which correspond to active genes in S. Enteritidis, nine of which are also inactive in the host adapted S. Gallinarum and S. Choleraesuis serovars. Sequencing of these 9 CDS in several S. Dublin clinical isolates revealed that they are pseudogenes in all of them, indicating that this feature is not peculiar to the sequenced strain. Among these CDS, shdA (Peyer´s patch colonization factor) and mglA (galactoside transport ATP binding protein), appear also to be inactive in the human adapted S. Typhi and S. Paratyphi A, suggesting that functionality of these genes may be relevant for the capacity of certain Salmonella serovars to infect a broad range of hosts.