Domperidone induces a probenecid-sensitive rise in immunoreactive prolactin in cerebroventricular perfusates in female rats.
Domperidone induces a probenecid-sensitive rise in immunoreactive prolactin in cerebroventricular perfusates in female rats.
复制标题
多潘立酮诱导雌性大鼠脑室灌注液中免疫反应性催乳素的丙磺舒敏感性升高。
DOI：
10.1016/0006-8993(92)90122-p
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Bridges,RS
中科院分区：
文献类型：
--
作者：
Felicio,LF;Bridges,RS
本研究通过监测多巴胺拮抗剂多潘立酮（DOMP）治疗的卵巢切除（OVX）大鼠血液和脑室灌注液中的ir-PRL水平，研究了免疫反应性催乳素（ir-PRL）进入脑脊液（CSF）的途径。在Expt. 1在用DOMP i. p.（2.5、5.0和10.0 mg/kg）处理的大鼠中测量PRL血浆水平。所有剂量的DOMP均显著增加PRL血浆水平。在Expt.用DOMP（10 mg/kg）或DOMP加主动转运阻断剂丙磺舒（PROB; 250 mg/kg）腹膜内处理2只动物。分别使用导管和推拉式灌注系统在单独的动物组中测量脑室灌注液中的血浆PRL和ir-PRL。DOMP诱导血浆PRL增加，30-40分钟后心室灌注液ir-PRL水平升高。PROB治疗诱导DOMP治疗的动物血浆PRL水平的增加更大，但延迟DOMP诱导的ir-PRL心室灌注液ir-PRL水平的增加。在用DOMP加PROB处理的大鼠中观察到的心室灌注液中ir-PRL升高的延迟可能是由于PROB干扰PRL从血液转运到CSF中。这些结果表明，在某些情况下，在CSF中的ir-PRL来源于垂体。
The present study examined immunoreactive prolactin (ir-PRL) access into the cerebrospinal fluid (CSF) by monitoring ir-PRL levels in the blood and cerebroventricular perfusates of ovariectomized (ovx) rats treated with the dopamine antagonist, domperidone (DOMP). In Expt. 1 PRL plasma levels were measured in rats treated with DOMP i.p. (2.5, 5.0 and 10.0 mg/kg). All doses of DOMP significantly increased PRL plasma levels. In Expt. 2 animals were treated i.p. with DOMP (10 mg/kg) or DOMP plus the active transport blocking agent, probenecid (PROB; 250 mg/kg). Plasma PRL and ir-PRL in cerebroventricular perfusates were measured in separate sets of animals using catheters and a push-pull perfusion system, respectively. DOMP induced an increase in plasma PRL that was followed 30–40 min later by a rise in ventricular perfusates ir-PRL levels. PROB treatment induced a greater increase in plasma PRL levels in DOMP-treated animals, but delayed the DOMP-induced increase in ir-PRL ventricular perfusate ir-PRL levels. The delay in the rise of ir-PRL in ventricular perfusates observed in rats treated with DOMP plus PROB may be due to a PROB's interference with the transport of PRL from the blood into CSF. These results suggest that under some conditions ir-PRL in CSF originates from the pituitary.