Detection and typing of cutaneous human papillomavirus types-A comparison of three different methods
Detection and typing of cutaneous human papillomavirus types-A comparison of three different methods
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DOI：
10.1016/j.jviromet.2012.10.010
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发表时间：
2013-05-01
影响因子：
3.1
通讯作者：
Nindl, Ingo
中科院分区：
文献类型：
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作者：
Antonsson, Annika;Michael, Kristina M.;Nindl, Ingo
皮肤人乳头瘤病毒(HPV)可能在皮肤鳞状细胞癌的发生发展中起一定作用。HPV在皮肤鳞状细胞癌中的拷贝数非常低，因此敏感和可靠的检测方法很重要，特别是在检查皮肤HPV的自然病史方面。在本研究中，检测了194份皮肤拭子和91份皮肤拭子中HPV型的存在，并用三种不同的基于聚合酶链式反应的方法进行了比较：(I)β/伽马皮肤HPVPCR反向斑点杂交(BGC-PCRIB)，(Ii)多重皮肤乳头瘤病毒基因分型(McPG)和(Iii)FAP聚合酶链式反应。BGC-PCRRLB法HPV感染率为75%(68/91)，McPG法为72.0%(124/194)，FAP-PCR法为72.0%(139/194)。在91份标本中，三种方法检测HPV的符合率分别为73%(66/91；kappa=0.34)、75%(68/91；kappa=0.32)和69%(63/91；kappa=0.25)。McPG和PAP共检测194例标本，总符合率为66%(129/194；kappa=0.24)。这三种方法之间的一致性是中等的，这可以解释为每种方法都可以检测到不同的HPV类型；多重感染的数量较多，而人体皮肤中的病毒复制数量较低。总体而言，在人类皮肤拭子中发现了多种类型的皮肤HPV。(C)2012爱思唯尔B.V.保留所有权利。
Cutaneous human papillomavirus (HPV) may play a role in the development of cutaneous squamous cell carcinoma. HPV copy numbers in cutaneous squamous cell carcinoma are very low and hence sensitive and reliable detection methods are important, particularly to examine the natural history of cutaneous HPV. In the present study, the presence of cutaneous HPV types was examined in 194 skin swabs and in a subgroup of 91 skin swabs, and compared using three different PCR based methods: (i) beta/gamma cutaneous HPV PCR reverse-line-blotting (BGC-PCR RIB), (ii) multiplex cutaneous papillomavirus genotyping (McPG) and (iii) FAP PCR. The HPV prevalence was 75% (68/91) with BGC-PCR RLB, 64% (124/194) with McPG and 72% (139/194) with FAP PCR. The agreement for the detection of HPV between the three methods in the subset of 91 samples was 73% (66/91; kappa = 0.34) for BGC-PCR RLB and McPG, 75% (68/91; kappa = 0.32) for BGC-PCR RLB and FAP PCR, and 69% (63/91; kappa = 0.25) for McPG and FAP PCR. For McPG and PAP PCR, 194 specimens were tested in total, with an overall agreement of 66% (129/194; kappa = 0.24) for the detection of HPV. The concordance between the three methods was moderate, which could be explained by different HPV types detectable with each method; the high number of multiple infections and the low viral copy number in human skin. Overall, many cutaneous HPV types were identified and multiple HPV types were found frequently in the human skin swabs. (c) 2012 Elsevier B.V. All rights reserved.