Monocarboxylate transporter expression at the onset of skeletal muscle regeneration.
Monocarboxylate transporter expression at the onset of skeletal muscle regeneration.
复制标题
骨骼肌再生开始时单羧酸转运蛋白的表达。
DOI：
10.1002/phy2.75
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发表时间：
2013
影响因子：
2.5
通讯作者：
Bottje,Walter
中科院分区：
文献类型：
--
作者：
Washington,TyroneA;Brown,Lemuel;Smith,DameonA;Davis,Gina;Baum,Jamie;Bottje,Walter
骨骼肌再生的开始以成肌细胞增殖为特征。增殖的成肌细胞有增加的能量需求，肌膜上的乳酸交换可以用来解决这种增加的需求。单羧酸转运蛋白（mct）参与乳酸盐在肌膜上的转运，并受到各种生理刺激的影响。然而，MCT在骨骼肌再生开始时的表达尚未确定。本研究的目的是确定骨骼肌再生是否改变了再生胫骨前肌（TA）中MCT的表达。雄性C57/BL6小鼠随机分为对照组（未损伤组）和布比卡因组（损伤组）。注射后3 d，提取TA，测定蛋白和基因表达。肌肉质量与胫骨长度之比减少21%（2.4±0.1 mg/mm vs. 1.9±0.2 mg/mm，P< 0.02）。注射布比卡因3天后，IGF‐1和MyoD基因表达分别增加了5.0倍（P< 0.05）和3.5倍（P< 0.05）。MCT‐1蛋白降低32% (P< 0.03)；然而，MCT‐1基因表达没有改变。MCT4蛋白及基因表达均无差异。乳酸脱氢酶（LDH）‐A蛋白表达增加71% （P< 0.0004）。注射布比卡因3天后，LDH‐B和线粒体酶细胞色素C氧化酶亚基的蛋白水平下降。CD147和PKC‐θ蛋白分别增加64% （P< 0.03）和79% （P< 0.02）。MCT1而非MCT4的表达在骨骼肌再生开始时发生改变，可能是为了调节骨骼肌细胞对乳酸的摄取和使用。
The onset of skeletal muscle regeneration is characterized by proliferating myoblasts. Proliferating myoblasts have an increased energy demand and lactate exchange across the sarcolemma can be used to address this increased demand. Monocarboxylate transporters (MCTs) are involved in lactate transport across the sarcolemma and are known to be affected by various physiological stimuli. However, MCT expression at the onset of skeletal muscle regeneration has not been determined. The purpose of this study was to determine if skeletal muscle regeneration altered MCT expression in regenerating tibialis anterior (TA) muscle. Male C57/BL6 mice were randomly assigned to either a control (uninjured) or bupivacaine (injured) group. Three days post injection, the TA was extracted for determination of protein and gene expression. A 21% decrease in muscle mass to tibia length (2.4 ± 0.1 mg/mm vs. 1.9 ± 0.2 mg/mm,P< 0.02) was observed. IGF‐1 and MyoD gene expression increased 5.0‐fold (P< 0.05) and 3.5‐fold (P< 0.05), respectively, 3 days post bupivacaine injection. MCT‐1 protein was decreased 32% (P< 0.03); however, MCT‐1 gene expression was not altered. There was no difference in MCT4 protein or gene expression. Lactate dehydrogenase (LDH)‐A protein expression increased 71% (P< 0.0004). Protein levels of LDH‐B and mitochondrial enzyme cytochrome C oxidase subunit decreased 3 days post bupivacaine injection. CD147 and PKC‐θ protein increased 64% (P< 0.03) and 79% (P< 0.02), respectively. MCT1 but not MCT4 expression is altered at the onset of skeletal muscle regeneration possibly in an attempt to regulate lactate uptake and use by skeletal muscle cells.