Overexpression of PtADC confers enhanced dehydration and drought tolerance in transgenic tobacco and tomato: effect on ROS elimination.
Overexpression of PtADC confers enhanced dehydration and drought tolerance in transgenic tobacco and tomato: effect on ROS elimination.
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DOI：
10.1016/j.bbrc.2011.08.015
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发表时间：
2011-09
影响因子：
3.1
通讯作者：
Bao-Quan Wang;Qinghua Zhang;Ji-Hong Liu;Guohuai Li
中科院分区：
文献类型：
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作者：
Bao-Quan Wang;Qinghua Zhang;Ji-Hong Liu;Guohuai Li
干旱是限制植物生长和生产力的主要环境因素。多胺已被证明作为胁迫分子，在植物适应非生物胁迫中积累。本研究将从枳中分离的精氨酸脱羧酶基因PtADC导入烟草和番茄中，研究其抗旱性。我们证明了转基因植物表现出脱水和耐旱性的改善。在脱水胁迫条件下，转基因株系中活性氧（ROS）的积累显著低于野生型。此外，在转基因烟草株系中，三个胁迫响应基因的转录水平都有所提高。综上所述，我们的研究结果表明，PtADC在耐旱性中起着关键作用，这至少部分归因于其在ROS解毒中的作用。
Drought is a major environmental factor that limits plant growth and productivity. Polyamines have been shown to act as stress molecules that accumulate in plant adaptation to abiotic stresses. In this study, an arginine decarboxylase gene isolated from Poncirus trifoliata, PtADC, was introduced into tobacco and tomato to investigate its function in drought tolerance. We demonstrate that the transgenic plants showed an improvement in dehydration and drought tolerance. Under dehydration stress conditions, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines as compared with the wild type. Moreover, the transcript levels of three stress-responsive genes were increased in the transgenic tobacco lines. Taken together, our results suggest that PtADC plays a key role in drought tolerance, which is, at least partially, attributed to its role in ROS detoxification.