Binding sites of HeLa cell nuclear proteins on the upstream region of adenovirus type 5 E1A gene.
Binding sites of HeLa cell nuclear proteins on the upstream region of adenovirus type 5 E1A gene.
复制标题
HeLa 细胞核蛋白在 5 型腺病毒 E1A 基因上游区域的结合位点。
DOI：
--
复制
发表时间：
1989
影响因子：
14.9
通讯作者：
Kei Fujinaga
中科院分区：
文献类型：
--
作者：
Koichi Yoshida;Mitsuo Narita;Kei Fujinaga
应用DNase I足迹分析，在5型腺病毒E1a基因上游区鉴定出21个HeLa细胞核蛋白结合位点。近端启动子区域包含5个结合位点，它们与E1a帽部位重叠，包括TATA盒、类TATA序列、CCAAT盒和-100区(+1)。-190区域是NFIII和OBP100等八聚体基序结合蛋白的潜在位点。E1A增强子元件1的上游拷贝是具有XGGAYGT(X=A，C；Y=A，T)结合特异性的因子(E1A-F)的位点。E1a-F因子还与另外三个位点结合，其中一个与远端的E1a增强子元件重合。远端元件还含有ATF因子的潜在位点。腺病毒DNA复制的最小起始点竞争CCAAT和TATA盒区域以及-190区域的DNA-蛋白质复合体的形成，表明这些区域与共同或相关的因子相互作用。
Twenty one binding sites of HeLa cell nuclear proteins were identified on the upstream region of adenovirus type 5 E1A gene using DNase I footprint assay. The proximal promoter region contained five binding sites that overlapped the cap site, TATA box, TATA-like sequence, CCAAT box, and -100 region relative to the E1A cap site(+1). The -190 region was a potential site for octamer-motif binding proteins, such as NFIII and OBP100. An upstream copy of the E1A enhancer element 1 was the site for a factor (E1A-F) with the binding specificity of XGGAYGT (X = A, C; Y = A, T). E1A-F factor also bound to three other sites, one of which coincided with the distal E1A enhancer element. The distal element also contained a potential site for ATF factor. The adenovirus minimal origin of DNA replication competed for DNA-protein complex formation on the CCAAT and TATA box region and the -190 region, suggesting that these regions interacted with a common or related factor.