Anesthesia effects on the low frequency blood flow oscillations in mouse skin
Anesthesia effects on the low frequency blood flow oscillations in mouse skin
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DOI：
10.1111/srt.12593
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发表时间：
2019-01-01
影响因子：
2.2
通讯作者：
Tankanag, A., V
中科院分区：
文献类型：
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作者：
Astashev, M. E.;Serov, D. A.;Tankanag, A., V
背景 当使用实验动物时，需要在记录前将其麻醉。然而，麻醉对动物血流振荡的影响尚未研究。使用激光多普勒血流计（LDF）技术研究了两种麻醉方式（唑来特-赛拉嗪和唑来特-一氧化二氮混合物）对小鼠皮肤灌注的影响。方法使用BALB/c小鼠。 LDF 探针置于左后爪的腹侧表面。使用连续自适应小波变换对 LDF 信号进行频谱分析，以识别和描述小鼠皮肤中的外周血流振荡。结果小鼠的低频振荡区间边界（肌源性、神经源性和内皮性）与人类和大鼠确定的边界一致，这表明它们与体型无关。与唑来特-笑气麻醉相比，唑来特-赛拉嗪麻醉分别显着降低神经源性和内皮振荡幅度 29% 和 50%，并使心脏振荡幅度增加 23%。与唑来特-赛拉嗪混合物相比，唑来特-一氧化二氮麻醉的肌源性和呼吸振荡幅度没有显着变化。结论 麻醉方式对皮肤血流振荡幅度的不同影响与唑来替赛拉嗪麻醉抑制交感神经活动有关。
Background When laboratory animals are used one needs to anesthetize them before recording. However, the influence of anesthesia on animal blood flow oscillations has not been studied. The effects of two ways of anesthesia, zoletil-xylazine, and zoletil-nitrous oxide mixtures, on mouse skin perfusion using laser Doppler flowmetry (LDF) technique were studied. Methods BALB/c mice were used. LDF probe was placed on the ventral surface of the left hind paw. Spectral analysis of LDF signals was performed with continuous adaptive wavelet transform to identify and describe peripheral blood flow oscillations in mouse skin. Results Low-frequency oscillation interval boundaries (myogenic, neurogenic, and endothelial) for mice were shown to coincide with the boundaries determined for human and rats, that demonstrate their independence from the body size. Zoletil-xylazine anesthesia significantly decreased neurogenic and endothelial oscillation amplitudes by 29% and 50% respectively and increased the amplitude of cardiac oscillations by 23% compared to zoletyl-nitrous oxide anesthesia. There were no significant changes of the amplitudes of myogenic and respiratory oscillations with zoletil-nitrous oxide anesthesia compared to the zoletil-xylazine mixture. Conclusion We suggest that the different influence of anesthesia modes on the amplitudes of skin blood flow oscillations is associated with sympathetic activity suppressed by zoletil-xylazine anesthesia.