Tissue specificity of enhancer and promoter activities of a HERV-K(HML-2) LTR
Tissue specificity of enhancer and promoter activities of a HERV-K(HML-2) LTR
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DOI：
10.1016/j.virusres.2004.02.036
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发表时间：
2004-08-01
期刊：
影响因子：
5
通讯作者：
Sverdlov, ED
中科院分区：
文献类型：
--
作者：
Ruda, VM;Akopov, SB;Sverdlov, ED
荧光素酶报告基因的瞬时表达用于评估人内源性逆转录病毒 K (HERV-K) 的孤立病毒外长末端重复序列 (LTR) 在几种人和 CHO 细胞系中的组织特异性启动子和增强子活性。 LTR 的启动子活性各不相同，从几乎不可检测（GS 和 Jurkat 细胞）到与 SV40 早期启动子（Tera-1 人睾丸胚胎癌细胞）一样高。 LTR 的负调控元件 (NRE) 在 LTR 可以充当启动子的所有细胞系中保留其活性。并且还能够结合宿主细胞核蛋白。 LTR 对 SV40 早期启动子的增强子活性仅在 Tera-1 细胞中检测到，而在不同来源的密切相关的人睾丸胚胎癌细胞系 NT2/D1 中未观察到。通过电泳迁移率变动分析对 Tera-1 和 NT2/D1 核提取物中与 LTR 中心部分结合的蛋白质进行比较，发现显着差异可以通过这些细胞中不同的 LTR 增强子活性来确定。 SV40 早期启动子活性的组织特异性也被揭示。 (C) 2004 Elsevier B.V. 保留所有权利。
Transient expression of a luciferase reporter gene was used to evaluate tissue-specific promoter and enhancer activities of a solitary extraviral long terminal repeat (LTR) of the human endogenous retrovirus K (HERV-K) in several human and CHO cell lines. The promoter activity of the LTR varied from virtually not detectable (GS and Jurkat cells) to as high as that of the SV40 early promoter (Tera-1 human testicular embryonal carcinoma cells). The negative regulatory element (NRE) of the LTR retained its activity in all cell lines where the LTR could act as a promoter. and was also capable of binding host cell nuclear proteins. The enhancer activity of the LTR towards the SV40 early promoter was detected only in Tera-1 cells and was not observed in a closely related human testicular embryonal carcinoma cell line of different origin, NT2/D1. A comparison of proteins bound to central part of the LTR in nuclear extracts from Tera-1 and NT2/D1 by electrophoretic mobility shift assay revealed striking differences that could be determined by different LTR enhancer activities in these cells. Tissue specificity of the SV40 early promoter activity was also revealed. (C) 2004 Elsevier B.V. All rights reserved.