Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells
Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells
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DOI：
10.1016/j.archoralbio.2010.07.023
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发表时间：
2010-12-01
影响因子：
3
通讯作者：
Sugiya, Hiroshi
中科院分区：
文献类型：
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作者：
Mitsui, Retsu;Fujita-Yoshigaki, Junko;Sugiya, Hiroshi
胰岛素样生长因子-I（IGF-I）在唾液腺中表达。我们研究了IGF-I对大鼠下颌下腺细胞数量、紧密连接蛋白的表达和分布以及细胞旁屏障功能的影响。当这些细胞在含有10%胎牛血清（FBS）或IGF-I的培养基中培养时，10天时细胞数量相当。然而，在IGF-I受体抑制剂的存在下，仅用FBS培养的细胞数量明显减少。紧密连接蛋白occludin和claudin-3在IGF-I或FBS培养的细胞中通过Western印迹类似地检测到。免疫组化显示，occludin和另一种紧密连接蛋白（ZO-1）类似地定位于IGF-I或FBS培养的细胞的细胞内连接。屏障功能通过跨上皮阻力（TER）和FITC-葡聚糖渗透性进行评价。用IGF-I或FBS培养的细胞的TER值和FITC-葡聚糖渗透性相当。这些观察结果表明，IGF-I不仅有助于维持唾液腺细胞的细胞数量，而且还通过紧密连接蛋白的表达和分布维持其细胞旁屏障功能。(C)2010爱思唯尔有限公司保留所有权利。
Insulin-like growth factor-I (IGF-I) is expressed in salivary glands. We examined the effects of IGF-I on cell number, the expression and distribution of tight junction proteins and the paracellular barrier function in cells derived from rat submandibular glands. When those cells were cultured in medium containing 10% foetal bovine serum (FBS) or IGF-I, the number of cells was comparable at 10 days. However, in the presence of inhibitor of IGF-I receptors, the number of cells cultured with FBS only was clearly reduced. The tight junction proteins occludin and claudin-3 were similarly detected by Western blotting in cells cultured with IGF-I or FBS. Immunostaining revealed that occludin and another tight junction protein (ZO-1) were similarly localized at intracellular junctions of cells cultured with IGF-I or FBS. The barrier functions were evaluated by transepithelial resistance (TER) and by FITC-dextran permeability. The TER values and FITC-dextran permeability of cells cultured with IGF-I or FBS were comparable. These observations suggest that IGF-I contributes to the maintenance not only of the cell number of salivary gland cells but also of their paracellular barrier function via the expression and distribution of tight junction proteins. (C) 2010 Elsevier Ltd. All rights reserved.