THE PROTO-ONCOGENE C-ETS IS PREFERENTIALLY EXPRESSED IN LYMPHOID-CELLS
THE PROTO-ONCOGENE C-ETS IS PREFERENTIALLY EXPRESSED IN LYMPHOID-CELLS
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DOI：
10.1128/mcb.5.11.2993
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
CHEN, JH
中科院分区：
文献类型：
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作者：
CHEN, JH
禽急性白血病病毒E26的转化序列包含两个不同的致癌基因，v-mybE和v-ets融合在一起。利用含有v-ets序列的探针，在禽组织中检测到c-ets原癌基因的聚腺苷化转录本；它们包括主要的7.0千碱基和次要的2.0千碱基物种。这些c-ets mrna仅在淋巴样器官和禽类T和B淋巴样细胞系中检测到高水平。在人造血细胞系中也观察到类似的c-ets转录模式，在淋巴样B细胞和T细胞中检测到转录本，但在红细胞或髓细胞中未检测到。将E26癌基因插入诱导表达载体，在细菌中产生90千道尔顿蛋白（bp90）。纯化bp90的兔抗血清沉淀了e26转化细胞中表达的v-mybE-ets多蛋白P135gag-mybE-ets，并与禽成髓细胞病病毒的转化蛋白p50v-mybA发生反应。用细菌合成的v-mybA蛋白吸收抗bp90血清，去除抗myb活性。吸收的抗bp90血清保留了从e -26转化细胞中免疫沉淀P135gag-mybE-ets的能力，并与鸡淋巴样器官中检测到的56千道尔顿多肽（p56）特异性反应，用于禽源和人源的T淋巴细胞和B淋巴细胞。这些数据表明p56是c-ets原癌基因的翻译产物，并暗示p56可能参与调节淋巴样细胞的生长。
The transforming sequences of the avian acute leukemia virus, E26, contain two distinct oncogenes, v-mybE and v-ets, fused together. By using a probe containing v-ets sequences, polyadenylated transcripts of the c-ets proto-oncogene were detected in avian tissues; they included a major 7.0-kilobase and a minor 2.0-kilobase species. These c-ets mRNAs were detected at high levels only in lymphoid organs and in avian T and B lymphoid cell lines. A similar pattern of c-ets transcription was observed in human hematopoietic cell lines, with transcripts detected in lymphoid B and T cells but not in erythroid or myeloid cells. The E26 oncogene was inserted into an inducible expression vector, and a 90-kilodalton protein (bp90) was produced in bacteria. Rabbit antisera raised to purified bp90 precipitated P135gag-mybE-ets, the v-mybE-ets polyprotein expressed in E26-transformed cells, and also reacted with p50v-mybA, the transforming protein of the avian myeloblastosis virus. Antiserum to bp90 was absorbed with a bacterially synthesized v-mybA protein to remove anti-myb activity. The absorbed anti-bp90 serum retained the ability to immunoprecipitate P135gag-mybE-ets from E-26-transformed cells and specifically reacted with a 56-kilodalton polypeptide (p56) detected in chicken lymphoid organs in T and B lymphocytes of both avian and human origin. The data suggest that p56 is a translational product of the c-ets proto-oncogene and imply that p56 may be involved in regulating the growth of lymphoid cells.