Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium
Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium
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使用化学成分确定的培养基在环境温度下短期保存猪受精卵
DOI：
10.1111/asj.13711
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发表时间：
2022
影响因子：
2
通讯作者：
Otoi Takeshige
中科院分区：
文献类型：
--
作者：
Lin Qingyi;Le Quynh Anh;Takebayashi Koki;Hirata Maki;Tanihara Fuminori;Sawamoto Osamu;Kikuchi Takeshi;Otoi Takeshige
我们的目标是开发一种简单的方法来短期保存体外产生的猪受精卵，在25°C下保存长达2 天。首先，我们评估了三种保存液在25℃下保存猪受精卵24 h的效果，其中两种是商用的细胞保存介质(CELL-W和CELL-S)，第三种是胎牛血清(FBS)。随后，我们研究了在25℃下将受精卵保存24-72 h的效果。在25℃下24 h保存效果最好，对囊胚质量没有明显影响。然而，短期保存24 h的猪受精卵与新鲜对照组相比，囊胚率降低。随着保存时间的延长，囊胚形成率从11.3%分别下降到4.4%和0%，随着保存时间的延长，囊胚形成率从11.3%显著降低到48或72 h。综上所述，在受精卵保存过程中，囊胚期的发育能力随时间的延长而降低。受精卵在化学定义的培养液中保存不会影响囊胚质量，但在100%的血清中保存会对发育中的胚胎产生不利影响，导致细胞凋亡。
We aimed to develop a simple method for the short‐term preservation of in vitro‐produced porcine zygotes at 25°C for up to 2 days. Firstly, we evaluated the efficiency of three storage solutions to preserve porcine zygotes at 25°C for 24 h. Two of these were commercially available defined media for cell storage (Cell‐W and Cell‐S), and the third was fetal bovine serum (FBS). Thereafter, we examined the effects of storing the zygotes in the Cell‐W solution for 24–72 h at 25°C. The Cell‐W solution was the most efficient for 24 h storage of porcine zygotes at 25°C, with no apparent effects on blastocyst quality. However, short‐term storage of porcine zygotes for 24 h reduced the blastocyst formation rate compared with the fresh control group. As storage duration increased from 24 to 48 or 72 h, blastocyst formation rates were significantly decreased from 11.3% to 4.4% and 0%, respectively. In conclusion, during zygote storage, the developmental competence to the blastocyst stage decreased with time. Storage of zygotes in chemically defined media did not affect blastocyst quality, but the storage in 100% serum had an adverse effect on developing embryos causing apoptosis.