Long-duration freewheel running and submandibular lymphocyte response to forced exercise in older mice
Long-duration freewheel running and submandibular lymphocyte response to forced exercise in older mice
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DOI：
10.1139/y06-011
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发表时间：
2006-05-01
影响因子：
2.1
通讯作者：
Hoffman-Goetz, L.
中科院分区：
文献类型：
--
作者：
Boudreau, J.;Hoffman-Goetz, L.
下颌下淋巴结（SLN）对于前房和上呼吸道的免疫监视至关重要;关于训练和运动如何影响SLN淋巴细胞知之甚少。本研究的目的是描述长期自由轮跑步后急性剧烈运动对小鼠SLN淋巴细胞的影响。雌性C57 BL/6小鼠被分配到跑步轮或保持久坐8个月，并进一步随机分配到跑步机运动和立即处死，跑步机运动和运动停止后24小时处死，或没有跑步机暴露。分离SLN淋巴细胞并分析CD 3、CD 4、CD 8和CD 19细胞表面标志物、作为细胞凋亡标志物的磷脂酰丝氨酸外化和作为氧化应激标志物的细胞内谷胱甘肽。与跑轮小鼠相比，老年久坐小鼠的T细胞百分比较低，B细胞百分比较高（p < 0.05）。尽管细胞内谷胱甘肽在各组之间没有差异，但跑步小鼠在跑步机运动后24小时，膜联蛋白V+ SLN淋巴细胞的百分比较低。还需要进一步的研究来确定自愿运动是否能改善前眼和上呼吸道的健康。
Submandibular lymph nodes (SLN) are crucial for immune surveillance of the anterior ocular chamber and upper respiratory tract; little is known about how training and exercise affect SLN lymphocytes. The intent of this study was to describe the impact of long term freewheel running followed by acute strenuous exercise on SLN lymphocytes in mice. Female C57BL/6 mice were assigned to running wheels or remained sedentary for 8 months, and further randomized to treadmill exercise and sacrifice immediately, treadmill exercise and sacrifice 24 h after exercise cessation, or no treadmill exposure. SLN lymphocytes were isolated and analyzed for CD3, CD4, CD8, and CD19 cell surface markers, phosphatidylserine externalization as a marker of apoptosis, and intracellular glutathione as a marker of oxidative stress. Compared with running wheel mice, older sedentary mice had a lower percent of T cells and higher percent of B cells (p < 0.05). Although intracellular glutathione did not differ between groups, running mice had a lower percent of Annexin V+ SLN lymphocytes 24 h after treadmill exercise. Further research will be needed to determine if voluntary exercise translates into improved anterior ocular and upper respiratory tract health.