Highly purified eicosapentaenoic acid increases interleukin-10 levels of peripheral blood monocytes in obese patients with dyslipidemia.
Highly purified eicosapentaenoic acid increases interleukin-10 levels of peripheral blood monocytes in obese patients with dyslipidemia.
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DOI：
10.2337/dc12-0269
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发表时间：
2012-12
期刊：
影响因子：
16.2
通讯作者：
Ogawa Y
中科院分区：
文献类型：
--
作者：
Satoh-Asahara N;Shimatsu A;Sasaki Y;Nakaoka H;Himeno A;Tochiya M;Kono S;Takaya T;Ono K;Wada H;Suganami T;Hasegawa K;Ogawa Y
最近已经强调，促炎（M1）巨噬细胞在肥胖症中占主导地位，而抗炎（M2）巨噬细胞占主导地位，从而导致肥胖症诱导的脂肪炎症和胰岛素抵抗。最近的一项临床试验表明，高纯度的二十碳五烯酸（EPA）可降低主要冠状动脉事件的发生率。在这项研究中，我们研究了EPA对肥胖血脂异常患者外周血单核细胞M1/M2样表型的影响。从26例无血脂异常的肥胖患者和90例有血脂异常的肥胖患者中制备外周血单核细胞。在后90例伴有血脂异常的肥胖患者中，82例患者接受或不接受EPA治疗（每日1.8 g），持续3个月。伴有血脂异常的肥胖患者的单核细胞中白细胞介素-10（IL-10）（一种M2标记物）的表达显著低于无血脂异常的患者。与对照组相比，EPA显著增加血清IL-10和EPA水平、EPA/花生四烯酸（AA）比值和单核细胞IL-10表达，并降低脉搏波速度（PWV）（动脉僵硬度指数）。EPA处理后，血清EPA/AA比值与单核细胞IL-10表达显著相关。只有单核细胞IL-10表达和血清脂联素的增加是EPA降低PWV的独立决定因素。EPA还通过过氧化物酶体增殖物激活受体（peroxisome proliferator-activated receptor，PPAR）γ依赖性途径显著增加人单核细胞THP-1细胞IL-10的表达和分泌。这项研究首次表明EPA增加单核细胞IL-10的表达与动脉硬度的降低平行，这可能有助于EPA在肥胖血脂异常患者中的抗动脉粥样硬化作用。
It has recently been highlighted that proinflammatory (M1) macrophages predominate over anti-inflammatory (M2) macrophages in obesity, thereby contributing to obesity-induced adipose inflammation and insulin resistance. A recent clinical trial revealed that highly purified eicosapentaenoic acid (EPA) reduces the incidence of major coronary events. In this study, we examined the effect of EPA on M1/M2-like phenotypes of peripheral blood monocytes in obese dyslipidemic patients. Peripheral blood monocytes were prepared from 26 obese patients without and 90 obese patients with dyslipidemia. Of the latter 90 obese patients with dyslipidemia, 82 patients were treated with or without EPA treatment (1.8 g daily) for 3 months. Monocytes in obese patients with dyslipidemia showed a significantly lower expression of interleukin-10 (IL-10), an M2 marker, than those without dyslipidemia. EPA significantly increased serum IL-10 and EPA levels, the EPA/arachidonic acid (AA) ratio, and monocyte IL-10 expression and decreased the pulse wave velocity (PWV), an index of arterial stiffness, compared with the control group. After EPA treatment, the serum EPA/AA ratio was significantly correlated with monocyte IL-10 expression. Only increases in monocyte IL-10 expression and serum adiponectin were independent determinants of a decreased PWV by EPA. Furthermore, EPA significantly increased the expression and secretion of IL-10 in human monocytic THP-1 cells through a peroxisome proliferator–activated receptor (PPAR)γ-dependent pathway. This study is the first to show that EPA increases the monocyte IL-10 expression in parallel with decrease of arterial stiffness, which may contribute to the antiatherogenic effect of EPA in obese dyslipidemic patients.
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影响因子：
29
作者：
Bouhlel, M. Amine;Derudas, Bruno;Chinetti-Gbaguidi, Giulia
通讯作者：
Chinetti-Gbaguidi, Giulia
DOI：
10.1161/atvbaha.106.136853
发表时间：
2007-09-01
影响因子：
8.7
作者：
Itoh, Michiko;Suganami, Takayoshi;Ogawa, Yoshihiro
通讯作者：
Ogawa, Yoshihiro
DOI：
10.1016/j.plefa.2008.07.002
发表时间：
2008-07-01
影响因子：
3
作者：
Kawashima, Akiko;Harada, Tsuyoshi;Mizuguchi, Kiyoshi
通讯作者：
Mizuguchi, Kiyoshi
DOI：
10.1161/01.atv.19.12.2847
发表时间：
1999-12-01
影响因子：
8.7
作者：
Oslund, LJP;Hedrick, CC;Fyfe, AI
通讯作者：
Fyfe, AI
影响因子：
4.8
作者：
Ohashi, Koji;Parker, Jennifer L.;Walsh, Kenneth
通讯作者：
Walsh, Kenneth