Rnq1: An epigenetic modifier of protein function in yeast
Rnq1: An epigenetic modifier of protein function in yeast
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DOI：
10.1016/s1097-2765(00)80412-8
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发表时间：
2000-01-01
期刊：
影响因子：
16
通讯作者：
Lindquist, S
中科院分区：
文献类型：
--
作者：
Sondheimer, N;Lindquist, S
已在酵母中鉴定出两种以蛋白质为基础的遗传元件(Prion)。目前还不清楚是否存在其他普恩病毒，也不知道如何找到它们。我们建立了在蛋白质数据库中搜索普恩候选基因的标准，并找到了几个候选基因。首先检测的是Rnq1，它以不同的、可遗传的物理状态存在，可溶和不溶。不溶状态占主导地位，并通过细胞质在细胞之间传递。当Rnq1的类Pron区域取代了[PSI+]的蛋白质决定簇Sup35的Prion结构域时，[PSI+]的表型和表观遗传行为被完全再现。这些发现将Rnq1确定为一种普恩病毒，证明了普恩蛋白结构域是模块化的和可转移的，并建立了一种识别和表征新的普恩蛋白的范例。
Two protein-based genetic elements (prions) have been identified in yeast. It is not clear whether other prions exist, nor is it understood how one might find them. We established criteria for searching protein databases for prion candidates and found several. The first examined, Rnq1, exists in distinct, heritable physical states, soluble and insoluble. The insoluble state is dominant and transmitted between cells through the cytoplasm. When the prion-like region of Rnq1 was substituted for the prion domain of Sup35, the protein determinant of the prion [PSI+], the phenotypic and epigenetic behavior of [PSI+] was fully recapitulated. These findings identify Rnq1 as a prion, demonstrate that prion domains are modular and transferable, and establish a paradigm for identifying and characterizing novel prions.