Continuous measurement of 14C-labeled carbon dioxide and lactic acid by cultured cells grown in Leighton tubes.
Continuous measurement of 14C-labeled carbon dioxide and lactic acid by cultured cells grown in Leighton tubes.
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通过在 Leighton 管中生长的培养细胞连续测量 14C 标记的二氧化碳和乳酸。
DOI：
10.1016/0003-2697(78)90764-9
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发表时间：
1978
影响因子：
2.9
通讯作者：
P. Brecher
中科院分区：
文献类型：
--
作者：
A. Tercyak;H. Kuan;L. Salcedo;P. Brecher
本文介绍了一种连续测定培养细胞在莱顿管中产生~（14）CO_2的方法。所开发的装置还允许在实验期间取孵育培养基的等分试样，用于分析释放到溶液中的标记代谢物。本文介绍了一种测定此类试样中[14 C]乳酸的简便方法。该装置的可重复性和实用性已被证明，通过培养成纤维细胞与葡萄糖标记的C-1或C-6的位置，并检查选定的药物对CO2和乳酸的生产的影响。
A procedure for continuous measurement of14CO2production by cultured cells grown in Leighton tubes has been described. The apparatus developed also permits aliquots of the incubation medium to be taken during the experiments for analysis of labeled metabolites released into the solution. A simple method for determination of [14C]lactic acid in such aliquots has been described. The reproducibility and usefulness of the apparatus has been demonstrated by incubating fibroblasts with glucose labeled in the C-1 or C-6 position, and examining the effects of selected drugs on CO2and lactic acid production.