Circulating naive and CD4+CD25high regulatory T cells in patients with autoimmune pancreatitis
Circulating naive and CD4+CD25high regulatory T cells in patients with autoimmune pancreatitis
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DOI：
10.1097/mpa.0b013e3181577553
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发表时间：
2008-03-01
期刊：
影响因子：
2.9
通讯作者：
Okazaki, Kazuichi
中科院分区：
文献类型：
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作者：
Miyoshi, Hideaki;Uchida, Kazushige;Okazaki, Kazuichi
目的：自身免疫性胰腺炎（AIP）是一种新的胰腺疾病。有几种免疫学和组织学异常的疾病，包括血清IgG 4水平的增加和淋巴细胞和IgG 4阳性浆细胞的浸润。IgG 4的作用尚不清楚。最近，调节性T细胞（Tcells）已被报道参与各种自身免疫性疾病的发展以及B细胞向产生IgG 4的浆细胞的转变。为了阐明TdR在AIP病理生理学中的作用，我们分析了AIP.Methods循环TdR：我们招募了27例AIP患者进行这项研究。为了比较，我们还招募了23名其他胰腺疾病患者和32名健康受试者作为对照。我们通过流式细胞术分析外周血中的T细胞亚群为CD 4（+）CD 25（高）和CD 4（+）CD 25（+）CD 45 RA（+）（初始）。AIP患者外周血CD 4 + CD 25 + T细胞亚群明显增高（3.01% +/- 1.77%），与酒精性慢性胰腺炎（CP）相比特发性CP组（1.53% ± 0.56%）与健康对照组（1.72% ± 0.81%）比较，差异有统计学意义（P < 0.05）。与健康对照组（0.83% +/- 0.65%）和CP组（酒精性和特发性CP; 0.52% +/-0.40%，P <0.05）相比，AIP组中的初始TdR显著降低（0.32%+/- 0.22%）。未经治疗的AIP患者中，CD 4（+）CD 25（高）Tregs数量与IgG 4相关（R = 0.53，P < 0.05）。结论：CD 4（+）CD 25（高）Tregs数量增加可能影响AIP中IgG 4的产生，而幼稚Tregs数量减少可能参与AIP的发病机制。
Objective: Autoimmune pancreatitis (AIP) is a new clinical entity of pancreatic disorder. There are several immunologic and histological abnormalities specific for the disease, including increased levels of serum IgG4 and infiltration of lymphocytes and IgG4-positive plasmacytes. The role of IgG4 is unclear. Recently, regulatory T cells (Tregs) have been reported to be involved in the development of various autoimmune diseases as well as B cell shifting to IgG4-producing plasmacytes. To clarify the role of Tregs in the pathophysiology of AIP, we analyzed circulating Tregs in AIP.Methods: We recruited 27 patients with AIP for this study. For comparison, we also recruited 23 patients with other pancreatic disease and 32 healthy subjects as controls. We analyzed Tregs as CD4(+)CD25(high) and CD4(+)CD25(+)CD45RA(+) (naive) from peripheral blood by flow cytometry.Results: In peripheral blood, CD4(+)CD25(high) Tregs were significantly increased in AIP patients (3.01% +/- 1.77%) compared with alcoholic chronic pancreatitis (CP) (1.65% +/- 0.58%), idiopathic CP (1.53% +/- 0.56%), and healthy control (1.72% +/- 0.81%, P < 0.05). Naive Tregs significantly decreased in AIP (0.32% +/- 0.22%) compared with healthy control (0.83% +/- 0.65%) and CP group (alcoholic and idiopathic CP; 0.52% +/- 0.40%, P G 0.05). In untreated AIP patients, the number of CD4(+)CD25(high) Tregs and IgG4 are correlated (R = 0.53, P < 0.05).Conclusions: Increased numbers of CD4(+)CD25(high) Tregs may influence IgG4 production in AIP, whereas decreased numbers of naive Tregs may be involved in the pathogenesis of AIP.