Multiple dosing of prostaglandin F2 alpha or epinephrine on cynomolgus monkey eyes. I. Aqueous humor dynamics.
Multiple dosing of prostaglandin F2 alpha or epinephrine on cynomolgus monkey eyes. I. Aqueous humor dynamics.
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发表时间：
1987-03
影响因子：
4.4
通讯作者：
C. Camras;S. Podos;J. Rosenthal;P. Lee;C. Severin
中科院分区：
文献类型：
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作者：
C. Camras;S. Podos;J. Rosenthal;P. Lee;C. Severin
在获得基线眼内压（IOP）测量值1周后，对于每种测试试剂，将前列腺素（PG）F2 α（250微克，在50微升盐水中）或肾上腺素2%溶液（50微升）每天两次局部施用至6只食蟹猴的一只眼睛，持续2周。两侧对照眼接受其各自的载体。PGF 2 α在首次给药后2 - 3小时开始显著降低IOP，此后持续。第一次给药后至少10小时和第六次给药后至少14小时仍存在显著（P <0.05）的肿胀效应。第7次给药后4小时，平均降低为基线以下10.2 +/- 3.5（+/- SD）mmHg（P <0.0025）。此时，对侧溶剂处理眼的IOP平均值也显著（P <0.01）降低，比基线低6.0 +/- 3.3（+/- SD）mmHg，这似乎不是继发于昼夜波动、重复眼压测量、实验操作或意外药物转移。在首次给药后3小时开始，肾上腺素显著（P <0.05）降低IOP，但这种降低程度很小且不一致。第5次给药后2至6小时，通过荧光光度法测量，PGF 2 α和肾上腺素均未改变房水流。由于测量时IOP通常过低，无法通过压陷眼压描记术评估流出道功能。而PGF 2 α并不改变瞳孔大小，肾上腺素引起显着的瞳孔扩张。（250字处删节）
After obtaining baseline intraocular pressure (IOP) measurements for 1 week, prostaglandin (PG) F2 alpha (250 micrograms in 50 microliters saline) or epinephrine 2% solution (50 microliters) was topically applied twice daily for 2 weeks to one eye of six cynomolgus monkeys for each agent tested. Contralateral control eyes received their respective vehicles. PGF2 alpha significantly reduced IOP beginning 2 to 3 hr after the first dose, persisting thereafter. A significant (P less than 0.05) hypotensive effect remained for at least 10 hr after the first dose and at least 14 hr after the sixth dose. At 4 hr after the seventh dose, the mean reduction was 10.2 +/- 3.5 (+/- SD) mmHg below baseline (P less than 0.0025). At this time, there was also a significant (P less than 0.01) mean reduction of IOP in the contralateral vehicle-treated eyes of 6.0 +/- 3.3 (+/- SD) mmHg below baseline, which did not appear to be secondary to diurnal fluctuations, repeated tonometry, experimental manipulation, or inadvertent drug transfer. Epinephrine significantly (P less than 0.05) reduced IOP beginning 3 hr after the first dose, but this reduction was minimal and not consistent. Neither PGF2 alpha nor epinephrine altered aqueous flow as measured by fluorophotometry 2 to 6 hr after the fifth dose. Outflow facility could not be assessed by indentation tonography because IOP was often too low at the time of measurement. Whereas PGF2 alpha did not alter pupil size, epinephrine caused significant pupillary dilation.(ABSTRACT TRUNCATED AT 250 WORDS)